Sentences with phrase «blood cells in culture»

They hit upon a small organic compound called SB 247464 that stimulated the growth of mouse white blood cells in culture and in living mice.

Not exact matches

The trophoblasts along with blood vessel cells were added to small dextran beads that were then spun around in a container filled with cell culture fluid, creating shear stress and rotational forces to better mimic the environment at the maternal - fetal interface than static cell - culture systems.

The team tested the technique on cells in culture that mimic the transformation of unspecialised white blood cells into full immune cells.

By analyzing chemical changes of the IRS - 2 protein in immortalized cultures of human white blood cells, it determined that IRS - 2 appeared in two different forms — «on,» which allows the signal to pass through, and «off,» which stops the signal from activating the cells into M2 macrophages.

Last spring the team reported that their fibers induced the formation of new blood vessels in both cell cultures and living animals.

In a previous related study published in the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collageIn a previous related study published in the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collagein the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collagein Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collagen.

They found that when IGF - 1 was added to the cultured blood cells there was increased lithium sensitivity only in the blood cells of those bipolar disorder patients who did not respond to lithium therapy.

The implications were hardly lost on the Bethesda crowd: If the virus was transmitted in cell cultures in Ruscetti's lab, it could also be contaminating the nation's blood supply as a result of blood donations from unknowingly infected donors.

To determine whether endothelial cells — the cells that line the interior surface of blood vessels — directly influence breast cancer cell growth, they then created unique organotypic models of lung and bone marrow microvascular niches, in which endothelial cells formed blood vessel - like structures in culture as they would in the original organ.

Interestingly, the outbreak strain grew at a normal pace in cultured human white blood cells, apparently by subverting the immune response to its benefit.

This soluble factor was found at higher levels in the blood of animals with lung tumors, could increase the activation of osteoblasts and contributed to the maturation of neutrophils in cultured cells.

All it took to make pluripotent stem cells, they reported, was briefly bathing blood cells from newborn mice in a mildly acidic solution and then tweaking the culture conditions.

It described the growth in cell cultures of the virus that causes AIDS, a breakthrough which led eventually to a blood test for HIV infection.

In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channeIn an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channein which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channein one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channein the adjacent channel.

For research purposes, these cells hold an important advantage over those from blood in that they can be cultured without the use of artificial stimulants, which can alter the cells» behavior.

Their method relied on briefly bathing blood cells from newborn mice in a mildly acidic solution and then tweaking culture conditions to produce stem cells.

Haruko Obokata of the RIKEN Center for Developmental Biology (CDB) in Kobe, Japan, and colleagues at other Japanese institutions and at Harvard Medical School in Boston reported that simply subjecting blood cells from newborn mice to a moderately acidic environment for 25 minutes and then tweaking culture conditions could generate pluripotent stem cells capable of developing into nearly all of a body's cell types.

Allender and former veterinary student Lauren Kane demonstrated, however, that administering treatments via a fine spray allows therapeutic levels of terbinafine, an antifungal agent that kills O. ophiodiicola in cell culture, to get into healthy snakes» blood plasma.

Following up on clues — including the fact that PMS is 56 percent heritable — the NIH researchers studied the genetic control of gene expression in cultured white blood cell lines from women with PMDD and controls.

The team's experiments in cell cultures, conducted with funding from an American Brain Tumor Association Discovery Grant, showed that coibamide A cuts off the cancer cells» ability to communicate with blood vessels and other cells, eventually starving the cell and triggering its death.

The cells were cultured in a suitable medium and the researchers checked the molecules they produced, in particular the pro-angiogenic ones, that is, molecules that nurture the production of new blood vessels.

Another, previously unknown gene — when the investigators worked out its protein's structure and studied it in tissue culture — proved to do just the opposite: The protein helped the schizont leave the infected blood cell.

They show that highly purified NS1 acts as a pathogen - associated molecular pattern (PAMP) that activates mouse macrophages and human peripheral blood mononuclear cells (PBMCs) in culture via TLR4, resulting in release of inflammatory cytokines — an effect that was blocked by either a TLR4 antagonist or an anti-TLR4 antibody.

Understanding and reproducing key features of bone marrow formation — and hence, the creation of blood cells and platelets in tissue culture for storage and later use — could help in treatment of a variety of medical problems.

Blood stem cell cultures: Blood stem cells from colonies (cell clusters) in vitro consisting of different blood cBlood stem cell cultures: Blood stem cells from colonies (cell clusters) in vitro consisting of different blood cBlood stem cells from colonies (cell clusters) in vitro consisting of different blood cblood cells.

In cultured human cells and in rabbits with implanted stents, Finn and colleagues showed that metformin augmented the effect of mTOR inhibitors on regrowth of the blood vessel lininIn cultured human cells and in rabbits with implanted stents, Finn and colleagues showed that metformin augmented the effect of mTOR inhibitors on regrowth of the blood vessel lininin rabbits with implanted stents, Finn and colleagues showed that metformin augmented the effect of mTOR inhibitors on regrowth of the blood vessel lining.

In a previous study by the same group 78, autologous culture - expanded MSC were infused to breast cancer patients to investigate whether MSC would enhance the engraftment of peripheral blood stem cells after myeloablative therapy.

These cells produced platelet - like particles (PLPs) similar to those isolated from human peripheral blood following an additional 18 days of culture in a chemically defined media without feeder cells.

Liposome - siRNA - peptide complexes cross the blood - brain barrier and significantly decrease PrPc on Neuronal cells and PrPRES in infected cell cultures.

The pharmacokinetic properties of J147 after a single dose of 20 mg / kg in mice show brain levels of about 600 nM at 2 h, over 10-fold above its EC50 in some cell culture neuroprotection assays [7], with a brain to blood ratio of approximately 0.5.

Delaney et al. [4] who have shown that cord blood - derived CD34 + cells cultured in StemSpan ™ medium with cytokines, on plates coated with Notch ligand, are expanded ex vivo while still maintaining their ability to engraft into immunocompromised mice.

Nakabayashi, Monk, and collaborators studied methylation patterns in adult and umbilical blood and placenta cells from healthy volunteers; brain tissue from a brain bank; and a cultured liver cell line.

Expansion of CD34 + cells normalized relative to the values obtained in SFEM medium (dark gray bar) after culturing purified CD34 + cord blood cells for 7 days in StemSpan ™ serum -[SFEM, SFEM II (gold bar)-RSB- and animal component - free ACF (orange bar) media, and six media from other suppliers (light gray bars).

In a curious twist resembling cellular alchemy, when blood T cells are cultured with lymphoid tissue from the tonsils or spleen, the blood T cells begin acting like lymphoid tissue T cells, becoming more activated and more susceptible to abortive infection and death by pyroptosis.

Umbilical cord blood can be harvested and the embryonic stem cells grown in culture.

It has two compartments, one to grow blood vessel cells in and another for cultured brain cells, mimicking the physiology of the BBB.

We came across a compound that had been isolated from a plant species and shown to have effects on blocking the growth of blood vessel cells, both in the culture dish and also in animal models.

This is basically tweaking the molecules: making small chemical changes to the compound in hopes of finding something that could block the growth of blood vessel cells initially in the culture dish without having toxic effects on other cell types.

BSRI has state - of - the - art equipment to support research related to blood and blood product safety in the areas of molecular biology, immunology, virology, tissue culture, cell processing and epidemiology

And while the human and T cells they studied in the laboratory were not specifically skin T cells they were isolated from mouse cell culture and from human blood — the skin has a large share of T cells in humans, he says, approximately twice the number circulating in the blood.

First, after culturing human peripheral blood mononuclear cells (hu - PBMCs) with the IL - 15 superagonist, flow cytometry revealed increases in granzyme B and perforin expression in the CD3 -, CD16 +, CD56 + NK cells.

After a very extended childhood spent dividing in a culture dish, even stem cells tend to grow up and assume adult roles as workaday nerve, muscle, or blood cells, never to return to their youthful state.

Our goal is to create a microfluidic 3D cell culture system representing a «placenta - on - a-chip» in order to mimic the nutrient / waste transfer between maternal blood and fetal blood that occurs in the cotyledon section of the placenta, and to test and observe the effects of ethanol within the maternal bloodstream and compare it to a similar in vivo situation.

Given the number of white blood cells in the urine, urinary culture would be useful but the white blood cells may be inflammatory in nature.

This condition is usually easily diagnosed by urine culture, though often signs of infection such as white blood cells or bacteria are actually visible in the urinalysis.

Unfortunately, we found that a soluble form of CD40L was not effective at activating and expanding canine B cells from peripheral blood lymphocytes in culture.

ACT - activated clotting time (bleeding disorders) ACTH - adrenocorticotropic hormone (adrenal gland function) Ag - antigen test for proteins specific to a disease causing organism or virus Alb - albumin (liver, kidney and intestinal disorders) Alk - Phos, ALP alkaline phosphatase (liver and adrenal disorders) Allergy Testing intradermal or blood antibody test for allergen hypersensitivity ALT - alanine aminotransferase (liver disorder) Amyl - amylase enzyme — non specific (pancreatitis) ANA - antinuclear antibody (systemic lupus erythematosus) Anaplasmosis Anaplasma spp. (tick - borne rickettsial disease) APTT - activated partial thromboplastin time (blood clotting ability) AST - aspartate aminotransferase (muscle and liver disorders) Band band cell — type of white blood cell Baso basophil — type of white blood cell Bile Acids digestive acids produced in the liver and stored in the gall bladder (liver function) Bili bilirubin (bile pigment responsible for jaundice from liver disease or RBC destruction) BP - blood pressure measurement BUN - blood urea nitrogen (kidney and liver function) Bx biopsy C & S aerobic / anaerobic bacterial culture and antibiotic sensitivity test (infection, drug selection) Ca +2 calcium ion — unbound calcium (parathyroid gland function) CBC - complete blood count (all circulating cells) Chol cholesterol (liver, thyroid disorders) CK, CPK creatine [phospho] kinase (muscle disease, heart disease) Cl - chloride ion — unbound chloride (hydration, blood pH) CO2 - carbon dioxide (blood pH) Contrast Radiograph x-ray image using injected radiopaque contrast media Cortisol hormone produced by the adrenal glands (adrenal gland function) Coomb's anti- red blood cell antibody test (immune - mediated hemolytic anemia) Crea creatinine (kidney function) CRT - capillary refill time (blood pressure, tissue perfusion) DTM - dermatophyte test medium (ringworm — dermatophytosis) EEG - electroencephalogram (brain function, epilepsy) Ehrlichia Ehrlichia spp. (tick - borne rickettsial disease) EKG, ECG - electrok [c] ardiogram (electrical heart activity, heart arryhthmia) Eos eosinophil — type of white blood cell Fecal, flotation, direct intestinal parasite exam FeLV Feline Leukemia Virus test FIA Feline Infectious Anemia: aka Feline Hemotrophic Mycoplasma, Haemobartonella felis test FIV Feline Immunodeficiency Virus test Fluorescein Stain fluorescein stain uptake of cornea (corneal ulceration) fT4, fT4ed, freeT4ed thyroxine hormone unbound by protein measured by equilibrium dialysis (thyroid function) GGT gamma - glutamyltranferase (liver disorders) Glob globulin (liver, immune system) Glu blood or urine glucose (diabetes mellitus) Gran granulocytes — subgroup of white blood cells Hb, Hgb hemoglobin — iron rich protein bound to red blood cells that carries oxygen (anemia, red cell mass) HCO3 - bicarbonate ion (blood pH) HCT, PCV, MHCT hematocrit, packed - cell volume, microhematocrit (hemoconcentration, dehydration, anemia) K + potassium ion — unbound potassium (kidney disorders, adrenal gland disorders) Lipa lipase enzyme — non specific (pancreatitis) LYME Borrelia spp. (tick - borne rickettsial disease) Lymph lymphocyte — type of white blood cell MCHC mean corpuscular hemoglobin concentration (anemia, iron deficiency) MCV mean corpuscular volume — average red cell size (anemia, iron deficiency) Mg +2 magnesium ion — unbound magnesium (diabetes, parathyroid function, malnutrition) MHCT, HCT, PCV microhematocrit, hematocrit, packed - cell volume (hemoconcentration, dehydration, anemia) MIC minimum inhibitory concentration — part of the C&S that determines antimicrobial selection Mono monocyte — type of white blood cell MRI magnetic resonance imaging (advanced tissue imaging) Na + sodium ion — unbound sodium (dehydration, adrenal gland disease) nRBC nucleated red blood cell — immature red blood cell (bone marrow damage, lead toxicity) PCV, HCT, MHCT packed - cell volume, hematocrit, microhematocrit (hemoconcentration, dehydration, anemia) PE physical examination pH urine pH (urinary tract infection, urolithiasis) Phos phosphorus (kidney disorders, ketoacidosis, parathyroid function) PLI pancreatic lipase immunoreactivity (pancreatitis) PLT platelet — cells involved in clotting (bleeding disorders) PT prothrombin time (bleeding disorders) PTH parathyroid hormone, parathormone (parathyroid function) Radiograph x-ray image RBC red blood cell count (anemia) REL Rocky Mountain Spotted Fever / Ehrlichia / Lyme combination test Retic reticulocyte — immature red blood cell (regenerative vs. non-regenerative anemia) RMSF Rocky Mountain Spotted Fever SAP serum alkaline phosphatase (liver disorders) Schirmer Tear Test tear production test (keratoconjunctivitis sicca — dry eye,) Seg segmented neutrophil — type of white blood cell USG Urine specific gravity (urine concentration, kidney function) spec cPL specific canine pancreatic lipase (pancreatitis)-- replaces the PLI test spec fPL specific feline pancreatic lipase (pancreatitis)-- replaces the PLI test T4 thyroxine hormone — total (thyroid gland function) TLI trypsin - like immunoreactivity (exocrine pancreatic insufficiency) TP total protein (hydration, liver disorders) TPR temperature / pulse / respirations (physical exam vital signs) Trig triglycerides (fat metabolism, liver disorders) TSH thyroid stimulating hormone (thyroid gland function) UA urinalysis (kidney function, urinary tract infection, diabetes) Urine Cortisol - Crea Ratio urine cortisol - creatine ratio (screening test for adrenal gland disease) Urine Protein - Crea Ratio urine protein - creatinine ratio (kidney disorders) VWF VonWillebrands factor (bleeding disorder) WBC white blood cell count (infection, inflammation, bone marrow suppression)

Performed Inventory and Procurement oversight and assisted and trained all students in activities such as Plasmid Construction, Elutriation of Whole Blood, Dendritic Cell Studies, Organ / Tissue Processing, Ctl Assays, Cell Culture Maintenance, Transfection, Animal Handling, Flow Cytometry and Microscopy.

Laboratory: Experience with laboratory applications in parasitology, immunology and molecular biology including, microscopy, BCA Protein Assay, ELISA, Flow Cytometry, Real Time qPCR, Luminex, Gas chromatography (GC) mass spectrometry, cotinine assays (saliva), magnetic bead cell separation (MACS, Percoll and Ficoll) and whole blood cultures.