Sentences with phrase «blot analyses with»

Western blot analyses with phospho - specific antibodies suggested that stretching induces phosphorylation of ERK of the MAP kinase pathway, but did not induce phosphorylation of phosphatidylinositol 3 - kinase.

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RNA blot analysis revealed a messenger RNA of four kilobases with highest amounts in brain and spleen.

(B) Southern blot analysis of EcoRI - digested genomic DNA from wt, Xpd † XPCS / wt, and Xpd † XP / wt recombinant embryonic stem cell clones hybridised with the 3 ′ probe depicted in (A).

(e, g) Western blot (WB) analysis of tyrosine phosphorylation (pTyr) and total Tie2 after Tie2 immunoprecipitation (IP) in BP (e) and HUVEC (g) upon stimulation with recombinant human (rh) Ang1 compared to unstimulated (us) cells.

(f) Western blot analysis of phosphorylated FOXO3A (pFOXO3A) and total FOXO3A in BP stimulated with rhAng1 or rhAng2 for 30 min.

(i, k) Western blot analysis of AKT phosphorylation (Ser473) in control (shCtr) and Tie2 - silenced (shTie2 I / shTie2 II) BP (i) and HUVEC (k) upon stimulation with rhAng1 or rhAng2.

Quantitative analysis of Snail and vimentin protein expression by Western blotting revealed a 3 - to 4-fold increase in nuclear Snail expression and an 8 - to 9-fold increase in vimentin expression in the MDA - MB 231 F3 and BCM2 F3 cells compared with the parental and hypoxia - exposed A3 subpopulations (Figure 6c).

Western blot analysis showed almost complete silencing of AKT1 gene product that was associated with decreased levels of p - mTOR (C).

Western blot analysis revealed high levels of p - mTOR associated with high levels of p - p70S6K, p - rpS6, p - 4E - BP1, and total eIF4E in both cell lines (Fig. 1).

To examine whether lethal toxin modulated JAK2 and / or STAT3 signaling, we next treated MNK - 3 cells with lethal toxin for 2 hr, stimulated with IL - 23 for the indicated time and then examined JAK2 and STAT3 phosphorylation by western blot analyses.

DNA methylation analyses using the bisulphite genomic sequencing method and Southern blotting indicated that the upstream boundary of the potential imprint may coincide with the 5» end of the upstream repeats.

(E) 106 BaF3 cells stably transduced with JAK2 V617F, Y931C and V617F / Y931C double JAK2 mutant were treated for 30 min with 300 nM CMP6 inhibitor or with DMSO as a control (− condition), lysed and subjected to Western blot analysis.

(B) BaF3 cells stably transduced with V617F, Y931C or V617F / Y931C mutant were lysed and subjected to Western blot analysis.

Twelve hours after transfection, cells were treated with or without IFNα for another 24 h. Cell lysates (Input) were then immunoprecipitated (IP) with anti — IRF - 9 antibody followed by Western blot (WB) analysis with anti-STAT2 antibody.

(A) 106 BaF3 Aut (V658I), Aut (F958V) and BaF3 cells stably transduced with M - RAS or (B) JAK1 V658I and F958V mutants were treated for 30 min with 500 nM CMP6 inhibitor or with 0.1 % DMSO as a control (− condition), lysed and subjected to Western blot analysis.

Laboratory activities minimally include performing a lab - based patient - oriented research project applicable to cancer genomics, with an emphasis on mastering techniques commonly used within a human genetics laboratory, such as PCR, gene sequencing, mutation analysis, western blots, and functional protein studies.

The recombinant strain, together with analysis of protein expression (i.e. western blot, or dot plot, or FACS analysis).

(F) Undifferentiated P19 cells, untreated or treated with 10 uM MG - 132 for 1 hour, followed by western blot analysis.

(D) Differentiated Ara - C treated P19 cells were treated with indicated doses of proteasome inhibitors, followed by western blot analysis of Oct4 protein expression.

(E) Luciferase assay and western blot analysis of 3T3 cells following transfection with PORE reporter and overnight treatment with indicated cAMP analogues.