Sentences with phrase «blot analysis after»
A, Western blot analysis after subcellular fractionation showed that Karpas 299 and SU - DHL1 cells express phosphorylated mTOR, 4E - BP1, and rpS6 as well as total eIF4E predominantly in the cytoplasm.
http://cancerres.aacrjournals.org/ Not exact matches
After three rounds, the efficiency of depletion was tested by Western blot analysis using C - terminal (23 — 37)-- specific anti — hIAPP antibody.
(e, g) Western blot (WB) analysis of tyrosine phosphorylation (pTyr) and total Tie2 after Tie2 immunoprecipitation (IP) in BP (e) and HUVEC (g) upon stimulation with recombinant human (rh) Ang1 compared to unstimulated (us) cells.
For Western blot assay and immunoprecipitation analysis, CD4 + T cells were isolated from mice spleen on day 21 after transplantation using a Mouse CD4 + T Cell Isolation Kit (Miltenyi Biotec), and the procedures were performed as described previously (35).
Twelve hours after transfection, cells were treated with or without IFNα for another 24 h. Cell lysates (Input) were then immunoprecipitated (IP) with anti — IRF - 9 antibody followed by Western blot (WB) analysis with anti-STAT2 antibody.