Sentences with phrase «blot analysis confirmed»

Western blot analysis confirmed adequate inhibition of protein expression in transiently transfected cells.

Western blot analysis confirmed that this was indeed the case.

Not exact matches

These results were also confirmed by Western blot analysis (Fig. 2C).

Northern blotting analyses were performed on control and 3 h hypoxic samples to confirm that mRNA levels for P0 remained unchanged during hypoxia.

Western blot and FACS analyses of isolated BP from WT and Tie2PEKO mice confirmed reduced Tie2 protein expression in pericytes (Supplementary Fig. 7).

Expression of adeno - myrAkt in infected cells was confirmed by Western blot analysis using the anti-HA antibody.

Expression of adeno - myrAkt in infected cells was confirmed by Western blot analysis using a monoclonal antibody specific for the HA tag.

To confirm whether ILP accumulation in the insulin - producing cells actually results in a decrease of ILP2 concentration in the circulating hemolymph, we collected hemolymph from wild - type, and gbp1, gbp2 ex67 heterozygous and homozygous mutant larvae at 24 h AL3E, and performed western blot analyses using an anti-ILP2 antibody.

To further increase the sensitivity and specificity of the PCR assays, amplified gag and pol sequences were confirmed by Southern blot analysis using the biotinylated oligoprobes XGAGP2 (5» ACC TTG CAG CAC TGG GGA GAT GTC 3»), and XPOLP (5» TTG ATG AGG CAC TGC ACA GAG ACC 3») and chemiluminescence detection.

ER - α was transiently overexpressed in 231 and 468 cells using the V16 - ERα plasmid (Addgene # 11351) and expression was confirmed by Western blot analysis (Fig. 4A).

The researchers used Western Blot analysis to confirm the proteomic results.