Sentences with phrase «blot analysis in»

The BIOMED - 2 multiplex PCR approach is a rapid and reliable procedure that is more sensitive than that of the Southern blot analysis in detecting clonality.

Using a 2 - mm micropunch (Harris Uni-core; EMS, Hatfield, Pennsylvania), tissue was collected from 2 major components of the medullary 5 - HT system, the raphé obscurus and paragigantocellularis lateralis (PGCL), according to the atlas of Paxinos and Huang, 19 and standardized protein samples were obtained for Western blot analysis in each SIDS case and control.20 Twenty - micrometer tissue sections were collected from the remaining blocks in a standardized manner for tissue receptor autoradiography.

BIOMED - 2 multiplex immunoglobulin / T - cell receptor polymerase chain reaction protocols can reliably replace Southern blot analysis in routine clonality diagnostics.

Not exact matches

Moreover, in situ hybridization and RNA blot analysis indicate that the 5HTlc receptor is expressed in neurons in many regions of the central nervous system and suggest that this subclass of receptor may mediate many of the central actions of serotonin.

RNA blot analysis revealed a messenger RNA of four kilobases with highest amounts in brain and spleen.

Northern analysis revealed that in three of these lines the transgene was expressed in skeletal muscles but not in any of the non-skeletal muscle tissues examined (Figure 1); in the fourth line, Z111B, the expression of the transgene was below the level of detection in these blots.

(B) Southern blot analysis of EcoRI - digested genomic DNA from wt, Xpd † XPCS / wt, and Xpd † XP / wt recombinant embryonic stem cell clones hybridised with the 3 ′ probe depicted in (A).

Western blot analysis of Beta - actin in various tissues and cell lines using Proteintech antibody 60008 -1-Ig at a dilution of 1:5000.

(e, g) Western blot (WB) analysis of tyrosine phosphorylation (pTyr) and total Tie2 after Tie2 immunoprecipitation (IP) in BP (e) and HUVEC (g) upon stimulation with recombinant human (rh) Ang1 compared to unstimulated (us) cells.

(f) Western blot analysis of phosphorylated FOXO3A (pFOXO3A) and total FOXO3A in BP stimulated with rhAng1 or rhAng2 for 30 min.

Western blot and FACS analyses of isolated BP from WT and Tie2PEKO mice confirmed reduced Tie2 protein expression in pericytes (Supplementary Fig. 7).

(i, k) Western blot analysis of AKT phosphorylation (Ser473) in control (shCtr) and Tie2 - silenced (shTie2 I / shTie2 II) BP (i) and HUVEC (k) upon stimulation with rhAng1 or rhAng2.

Data was kindly provided by Ann Hammarstedt (Department of Molecular and Clinical Medicine, Gothenburg University, Sweden), who performed the Western blot analysis of Akt proteins, incubation of cells in MCD - media, and subsequent qRT - PCR analysis.

Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004 -1-Ig at a dilution of 1:10000.

Expression of adeno - myrAkt in infected cells was confirmed by Western blot analysis using the anti-HA antibody.

Additionally, Western blot analysis indicated that the expression level of p21waf1 / cip1 gene was also enhanced by the ectopic expression of DDX3 in HuH - 7, HeLa, and 293T cells (Fig. 2B).

Quantitative analysis of Snail and vimentin protein expression by Western blotting revealed a 3 - to 4-fold increase in nuclear Snail expression and an 8 - to 9-fold increase in vimentin expression in the MDA - MB 231 F3 and BCM2 F3 cells compared with the parental and hypoxia - exposed A3 subpopulations (Figure 6c).

A, Western blot analysis after subcellular fractionation showed that Karpas 299 and SU - DHL1 cells express phosphorylated mTOR, 4E - BP1, and rpS6 as well as total eIF4E predominantly in the cytoplasm.

Western blot analysis confirmed adequate inhibition of protein expression in transiently transfected cells.

Expression of adeno - myrAkt in infected cells was confirmed by Western blot analysis using a monoclonal antibody specific for the HA tag.

Western blot analysis revealed high levels of p - mTOR associated with high levels of p - p70S6K, p - rpS6, p - 4E - BP1, and total eIF4E in both cell lines (Fig. 1).

Western Blot analysis of LGALS3 expression in human stomach.

Although Southern blot analysis has proved to be a gold standard method in the detection of clonal rearrangement, it has several technical disadvantages, such as time necessary to obtain results, the use of radioactivity, and the susceptibility of the method to various artifacts [5].

Western Blot analysis of LGALS3 expression in mouse brain.

Western Blot analysis of LGALS3 expression in Hela S3 NE.

We first assessed systemic levels of IIS signaling by performing western blot analyses to measure phospho - AKT levels in the wing discs.

DNA preparation, western blot, immunofluorescence, immunohistochemistry, isobolographic analysis, mRNA - seq library preparation and sequencing analysis, ChIP - seq library preparation and sequencing analysis, the TUNEL assay, murine model and micro-positron emission tomography and computed tomography imaging are described in the Online Supplementary Methods.

It seems that the Southern blot analysis can now be reliably replaced in a routine laboratory setting [6].

To confirm whether ILP accumulation in the insulin - producing cells actually results in a decrease of ILP2 concentration in the circulating hemolymph, we collected hemolymph from wild - type, and gbp1, gbp2 ex67 heterozygous and homozygous mutant larvae at 24 h AL3E, and performed western blot analyses using an anti-ILP2 antibody.

For Western blot analysis, 106 BaF3 cells were starved for 4 h and lysed in 250 μL of Laemmli buffer (BioRad).

By Western blot analysis, we first examined whether the Janus - activated kinase (JAK)- STAT pathway — activated ISGF3 complex was involved in IFNα - induced RIG - G up - regulation.

Western blot analysis indicated that the complemented clone ospC7 / ospC +4 synthesized OspC at a level comparable to the WT parental strain and was influenced by pH, as described in ref.

Western blot analysis showed that the COX - 2 protein levels were significantly decreased in the STAT6 DN cells compared to the parental or vector - containing cells (Figure 2a).

Additionally, southern blot analyses did not detect integration of plasmids in these clones (Fig. 3C).

ER - α was transiently overexpressed in 231 and 468 cells using the V16 - ERα plasmid (Addgene # 11351) and expression was confirmed by Western blot analysis (Fig. 4A).

Western blot analysis of UNC - 7 isoforms translated in rabbit reticulocytes.

(D) Western blot analysis showing that overexpression of PSD - 95 enhances BDNF - induced p - Akt and p - CaMKII, but not p - Erk or p - TrkB in SH - SY5Y - TrkB transfected cells.

(E) Western blot analysis showing that overexpression of PSD - 95 restores BDNF signaling in Ube3A RNAi knockdown SH - SY5Y cells.