Sentences with phrase «blot analysis indicate»

Moreover, in situ hybridization and RNA blot analysis indicate that the 5HTlc receptor is expressed in neurons in many regions of the central nervous system and suggest that this subclass of receptor may mediate many of the central actions of serotonin.

Additionally, Western blot analysis indicated that the expression level of p21waf1 / cip1 gene was also enhanced by the ectopic expression of DDX3 in HuH - 7, HeLa, and 293T cells (Fig. 2B).

Western blot analysis indicated that the complemented clone ospC7 / ospC +4 synthesized OspC at a level comparable to the WT parental strain and was influenced by pH, as described in ref.

Not exact matches

(E) Northern blot analysis of total RNA isolated from testis of homozygous wt and XpdTTD / TTD, heterozygous Xpd † XPCS / wt and XpdTTD / wt, and compound heterozygous Xpd † XPCS / TTD mice as indicated.

To examine whether lethal toxin modulated JAK2 and / or STAT3 signaling, we next treated MNK - 3 cells with lethal toxin for 2 hr, stimulated with IL - 23 for the indicated time and then examined JAK2 and STAT3 phosphorylation by western blot analyses.

Western blot analysis showed that phosphoinositide - dependent protein kinase 1, the upstream kinase that phosphorylates Akt, and several phosphatases (phosphatase and tensin homolog deleted on chromosome 10, protein phosphatase 1, and protein phosphatase 2A), known as negative regulators of the PI3K / Akt signaling pathway, were unchanged upon VPA treatment (Supplemental Fig. 4E), indicating that VPA might act on Akt directly.

DNA methylation analyses using the bisulphite genomic sequencing method and Southern blotting indicated that the upstream boundary of the potential imprint may coincide with the 5» end of the upstream repeats.

Relevant restriction sites used for cloning or for Southern blot analysis are indicated.

(D) Differentiated Ara - C treated P19 cells were treated with indicated doses of proteasome inhibitors, followed by western blot analysis of Oct4 protein expression.

(E) Luciferase assay and western blot analysis of 3T3 cells following transfection with PORE reporter and overnight treatment with indicated cAMP analogues.