Sentences with phrase «blot analysis using»

To further increase the sensitivity and specificity of the PCR assays, amplified gag and pol sequences were confirmed by Southern blot analysis using the biotinylated oligoprobes XGAGP2 (5» ACC TTG CAG CAC TGG GGA GAT GTC 3»), and XPOLP (5» TTG ATG AGG CAC TGC ACA GAG ACC 3») and chemiluminescence detection.

Expression of adeno - myrAkt in infected cells was confirmed by Western blot analysis using a monoclonal antibody specific for the HA tag.

Expression of adeno - myrAkt in infected cells was confirmed by Western blot analysis using the anti-HA antibody.

By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band.

After three rounds, the efficiency of depletion was tested by Western blot analysis using C - terminal (23 — 37)-- specific anti — hIAPP antibody.

To confirm whether ILP accumulation in the insulin - producing cells actually results in a decrease of ILP2 concentration in the circulating hemolymph, we collected hemolymph from wild - type, and gbp1, gbp2 ex67 heterozygous and homozygous mutant larvae at 24 h AL3E, and performed western blot analyses using an anti-ILP2 antibody.

Not exact matches

EdiGene Knockout Cell Lysates can be used to validate antibodies for specificity or used as high - quality negative controls for Western blot analysis.

Western blot analysis of Beta - actin in various tissues and cell lines using Proteintech antibody 60008 -1-Ig at a dilution of 1:5000.

Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004 -1-Ig at a dilution of 1:10000.

Western blot analysis was done using standard methods as described previously (21).

Although Southern blot analysis has proved to be a gold standard method in the detection of clonal rearrangement, it has several technical disadvantages, such as time necessary to obtain results, the use of radioactivity, and the susceptibility of the method to various artifacts [5].

Western blot analysis of chondroitin sulfate proteoglycans and Olig2 was performed as previously described [57], using anti-phosphacan monoclonal (1 ∶ 1000, 3F8, Developmental Studies Hybridoma Bank), anti-CSPG4 / NG2 monoclonal (1 ∶ 2000, Chemicon), anti-Olig2 polyclonal (1 ∶ 4000, Chemicon) and anti-b-tubulin monoclonal antibody (1 ∶ 1000, Santa Cruz).

Western blot analysis of antibody specificity has been done using a routine sample setup composed of IgG / HSA - depleted human plasma and protein lysates from a limited number of human tissues and cell lines.

For Western blot assay and immunoprecipitation analysis, CD4 + T cells were isolated from mice spleen on day 21 after transplantation using a Mouse CD4 + T Cell Isolation Kit (Miltenyi Biotec), and the procedures were performed as described previously (35).

Using a 2 - mm micropunch (Harris Uni-core; EMS, Hatfield, Pennsylvania), tissue was collected from 2 major components of the medullary 5 - HT system, the raphé obscurus and paragigantocellularis lateralis (PGCL), according to the atlas of Paxinos and Huang, 19 and standardized protein samples were obtained for Western blot analysis in each SIDS case and control.20 Twenty - micrometer tissue sections were collected from the remaining blocks in a standardized manner for tissue receptor autoradiography.

Anti-goat MSTN monoclonal antibody (1 ∶ 2000 dilution) and 1 ∶ 1000 dilution of a mouse anti-actin antibody (Sigma, A4700) were used for the western blot analysis.

DNA methylation analyses using the bisulphite genomic sequencing method and Southern blotting indicated that the upstream boundary of the potential imprint may coincide with the 5» end of the upstream repeats.

Relevant restriction sites used for cloning or for Southern blot analysis are indicated.

Laboratory activities minimally include performing a lab - based patient - oriented research project applicable to cancer genomics, with an emphasis on mastering techniques commonly used within a human genetics laboratory, such as PCR, gene sequencing, mutation analysis, western blots, and functional protein studies.

SRM - MS analysis will permit us to directly use small - sized samples, such as the individual CSF samples, enable verification of marker candidates that currently do not have available antibodies (hence not amenable to conventional analyses such as ELISA or Western blots), and provide robust statistical analyses on individual candidate markers or combinations of them to determine which would make the best biomarker (s) for a particular disease condition.

(E) Western blot analysis of iPS - RPE protein expression using antibodies against a panel of RPE cell markers.

ER - α was transiently overexpressed in 231 and 468 cells using the V16 - ERα plasmid (Addgene # 11351) and expression was confirmed by Western blot analysis (Fig. 4A).

The researchers used Western Blot analysis to confirm the proteomic results.

Western blot analysis of of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho - PDGF Receptor (Tyr754)(23B2) Rabbit.