Sentences with phrase «bp target sequences»
One ZFN pair was used to target both the human and rhesus macaque CXCR4 genes since the 24 bp target sequences are identical.
http://journals.plos.org/ Not exact matches
After four rounds of selection, the enriched oligo pool was sequenced, and a position weighted matrix was generated for the 12 bp target site and one flanking residue per side (faded).
It became possible to design FLEx vectors (Flip - excision vectors) that only allow one inversion event when it was discovered that it is not the exact sequence of the asymmetric target site core but its 8 bp length that is critical for Cre and FLP function.
Sequence coverage in regions immediately flanking our hybridization targets was in several cases higher than 8X, therefore, we extended SNP calling to 250 bp of flanking sequence in all Sequence coverage in regions immediately flanking our hybridization targets was in several cases higher than 8X, therefore, we extended SNP calling to 250 bp of flanking sequence in all sequence in all targets.
BLASTing the 15 - bp PORE sequence revealed a large cohort of genes potentially regulated by such a mechanism (although osteopontin, the one target gene previously known to be regulated by a PORE sequence, was not identified in this analysis).