Quantitative RT - PCR using RNAs extracted from
brain, thorax and abdomen of workers revealed that statistically significant and prominent Acks induction was observed only in the
brain when the bees were exposed to
high temperature, and scarce Acks expression was detected in the thorax and abdomen
under both room and
high temperatures (Figure 5O), further supporting that Acks induction
under a
high temperature reflects neural activity and does not result from some «heat shock responses» that could occur independently of neural activity.