Sentences with phrase «break at the target site»
The higher the amount of fluorescence, the better that gRNA is at causing a double stranded break at the target site, allowing the two halves of EGFP to recombine by homology directed repair, and resulting in the expression of EGFP.
http://blog.addgene.org/ Not exact matches
For editing the genome, this system makes use of 3 components, a guide RNA (gRNA) of about 125 nt that specifies the target, the Cas9 endonuclease that creates the DNA double - strand break (DSB) at the target site, and a donor oligonucleotide or plasmid as the repair material if needed (for knock in models).