The omega - 3 metabolites also reduced the ability to move in the triple - negative
breast cancer cell lines by 20 to 60 %.
These results provide the validity to the newly developed hypoxia / reoxygenation culture system for examining the regulation of CSCs in
breast cancer cell lines by niche factors in the tumor microenvironment and developing differential targeting strategies to eradicate breast CSCs.
Ab7291 staining alpha tubulin in human
breast cancer cell line by ICC / IF (Immunocytochemistry / Immunofluorescence).
Not exact matches
To see whether
cancer stem
cell renewal involves a chain of events similar to that used
by embryonic stem
cells, and whether the process was affected
by oxygen levels, Semenza and graduate student Chuanzhao Zhang focused their studies on two human
breast cancer cell lines that responded to low oxygen
by ramping up production of the protein ALKBH5, which removes methyl groups from mRNAs.
The researchers treated 63
cancer cell lines (26
breast, 14 colorectal and 23 ovarian) with low - dose 5 - azacitidine (AZA), an FDA - approved drug for myelodysplastic syndrome, that reverses epigenetic changes
by stripping off the methyl group that silences the gene.
The researchers then exposed
cells from each of these
lines to a panel of 31 different drug treatments — including 23 chemotherapy compounds approved
by the FDA for
breast and ovarian
cancers, six targeted
cancer drugs, and two common drug combinations.
Using a new TaqMan PCR - based technique, the researchers screened a number of
cancer cell lines from various tissues, and discovered that tRNA halves were specifically expressed in large quantities in sex hormone - dependent
cancers, i.e., estrogen receptor (ER)- positive
breast cancer and androgen receptor (AR)- positive prostate
cancer that are driven
by the hormones estrogen and testosterone.
Here, we show that a non-adherent, stem - like, and metastatic CSC - enriched subpopulation could be isolated
by exposing human metastatic
breast cancer cell lines to cycles of chronic hypoxia followed
by reoxygenation.
Using cutting - edge techniques enabled
by next - generation sequencing, the authors generated complete methylome maps at single nucleotide resolution in a low - passage
breast cancer cell line and normal
breast tissue (primary human mammary epithelial
cells).
Consistent with the demonstration that LARP7 is necessary for the integrity of the 7SK snRNA (He et al., 2008), the reduced LARP7 expression in invasive
breast cancer cell lines was accompanied
by a decrease in 7SK snRNA (Figure 1D).