Sentences with phrase «breast cancer cell lines from»

We purchased MDA - MB - 231 (MB231) and MCF7 breast cancer cell lines from American Type Culture Collection.

Not exact matches

Dr. Jochen Maurer and his research group were able to cultivate several cancer stem cell lines from triple receptor - negative breast cancer that are excellent representations of the original tumors they isolated from the patients.

This is a breast cancer stem cell line 1 (BCSC1) from the newly established cell model.

Beyond lung cancer, TiY is able to target TICs in 28 types of human cell lines derived from the central nervous system, melanoma, breast, renal, ovarian, colon, and prostate cancer.

To see whether cancer stem cell renewal involves a chain of events similar to that used by embryonic stem cells, and whether the process was affected by oxygen levels, Semenza and graduate student Chuanzhao Zhang focused their studies on two human breast cancer cell lines that responded to low oxygen by ramping up production of the protein ALKBH5, which removes methyl groups from mRNAs.

With help from researchers at Duke University, they showed that the gene has a unique pattern of methylation in breast cancer cell lines that might one day help doctors spot the disease.

A study combining tumor cells from patients with breast cancer with a laboratory model of blood vessel lining provides the most compelling evidence so far that a specific trio of cells is required for the spread of breast cancer.

The researchers have isolated the sesquiterpene lactone damsin from the plant and studied its effect on cancer stem cells in three different breast cancer cell lines.

Breast cancer researchers have mapped early genetic alterations in normal - looking cells at various distances from primary tumours to show how changes along the lining of mammary ducts can lead to disease.

The researchers then exposed cells from each of these lines to a panel of 31 different drug treatments — including 23 chemotherapy compounds approved by the FDA for breast and ovarian cancers, six targeted cancer drugs, and two common drug combinations.

Cheng, an assistant professor of medicine in hematology / oncology at Feinberg, provided the cell lines and NanoFlare targets the researchers used to model blood samples taken from breast cancer patients.

Using a new TaqMan PCR - based technique, the researchers screened a number of cancer cell lines from various tissues, and discovered that tRNA halves were specifically expressed in large quantities in sex hormone - dependent cancers, i.e., estrogen receptor (ER)- positive breast cancer and androgen receptor (AR)- positive prostate cancer that are driven by the hormones estrogen and testosterone.

They collected gene expression data from six genetically diverse breast cancer cell lines, which were each exposed to 109 small - molecule drugs at multiple dosages and time points.

Quantitative proteomics study of breast cancer cell lines isolated from a single patient: discovery of TIMM17A as a marker for breast cancer.

We then isolated and exposed this novel subpopulation to additional cycles of hypoxia / reoxygenation and established a distinct subpopulation of cells from these two breast cancer cell lines.

To evaluate whether ABL kinases might regulate the secretion of osteoblast - derived RANKL or OPG leading to osteoclast differentiation, we analyzed RANKL and OPG mRNA abundance in the murine osteoblast cell line 7F2 in response to conditioned medium from control and ABL1 / ABL2 knockdown breast cancer cells.

The human breast cancer cell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotcell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotCell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotics.

Whereas conditioned medium from ABL1 / ABL2 - depleted breast cancer cells did not affect RANKL abundance in osteoblasts compared with the cells treated with control conditioned medium (Fig. 5E), we found that conditioned medium from breast cancer cells lacking ABL kinases increased OPG abundance in the osteoblast cell line (Fig. 5F).

To confirm specificity, supernatant from transfected or transduced HB1.F3 cells was incubated with MCF7, MCF7 / HER2, or BT474, a human breast cancer cell line that endogenously overexpresses HER2.

The functional data provided from a limited series of breast cancer cell lines does not allow generalizable conclusions to be drawn.

Those are cells that were taken from nun many years ago that had breast cancer and they propagated them and they do a lot of the research on this line of cells that actually came from a nun that had breast cancer called MCF - 7 estrogen dependent breast cancer.