Sentences with phrase «breast cancer cell lines with»

To directly evaluate the relationship between ABL family kinases and metastasis, we analyzed ABL1 and ABL2 protein abundance in MDA - MB - 231 — derived breast cancer cell lines with different organ metastasis tropisms (19).

When Possemato suppressed PHGDH protein production in breast cancer cell lines with elevated levels of it, the cells stopped proliferating.

Not exact matches

This work is being done in collaboration with Dr. Hasan Korkaya, assistant professor, Biochemistry and Molecular Biology at the Medical College of Georgia, who has developed drug - resistant cell lines, and with breast cancer clinicians.

Banik cautions that neither of the contrast agents has yet been tested on animals injected with breast cancer cell lines, which he calls an important step to take.

The researchers treated 63 cancer cell lines (26 breast, 14 colorectal and 23 ovarian) with low - dose 5 - azacitidine (AZA), an FDA - approved drug for myelodysplastic syndrome, that reverses epigenetic changes by stripping off the methyl group that silences the gene.

With help from researchers at Duke University, they showed that the gene has a unique pattern of methylation in breast cancer cell lines that might one day help doctors spot the disease.

A study combining tumor cells from patients with breast cancer with a laboratory model of blood vessel lining provides the most compelling evidence so far that a specific trio of cells is required for the spread of breast cancer.

If a woman has an abortion, she's left with a large number of these immature cells lining her breast ducts, and she is therefore more vulnerable to cancer down the road — 30 percent more vulnerable, Brind says, than a woman who has never had an abortion.

In the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in mice.

Recent studies with human breast cancer cell lines have also shown that Shp2 mediates survival signals in cancer cells.

Using a qRT - PCR - based method, we found dramatic decreases of epithelial miRNAs (miR200c and miR205) in the F3 subpopulations compared with the parental breast cancer cell lines (Figure 6e).

Breast Cancer Cell Line Classification and Its Relevance with Breast Tumor Subtyping Xiaofeng Dai, Hongye Cheng, Zhonghu Bai, Jia Li Journal of Cancer 2017; 8:3131 - 3141

The human breast cancer cell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotcell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotCell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotics.

Whereas conditioned medium from ABL1 / ABL2 - depleted breast cancer cells did not affect RANKL abundance in osteoblasts compared with the cells treated with control conditioned medium (Fig. 5E), we found that conditioned medium from breast cancer cells lacking ABL kinases increased OPG abundance in the osteoblast cell line (Fig. 5F).

Breast cancer cell lines contain functional cancer stem cells with metastatic capacity and a distinct molecular signature.

Cultures of the human triple negative breast cancer cell line, HCC1806, were maintained in RPMI 1640 medium supplemented with 10 % FBS.

Although the differing effects of hypoxia reported here are in conflict with previously published data, where no contrasting effect was seen (18 — 20), our data was produced using 13 primary breast cancer samples, as well as established cell lines, and the opposing effects in hormone receptor — positive and negative tumors, measured using multiple in vitro and in vivo CSC assays, were consistent in all cases.

In collaboration with Nanyang Technological University, experiments were also conducted to co-deliver paclitaxel and small interfering RNA (siRNA) targeting a protein that prevents cell death (Bcl - 2) to MDA - MB - 231 human breast cancer cell line.

To confirm specificity, supernatant from transfected or transduced HB1.F3 cells was incubated with MCF7, MCF7 / HER2, or BT474, a human breast cancer cell line that endogenously overexpresses HER2.

Consistent with the demonstration that LARP7 is necessary for the integrity of the 7SK snRNA (He et al., 2008), the reduced LARP7 expression in invasive breast cancer cell lines was accompanied by a decrease in 7SK snRNA (Figure 1D).

For example, women with stage 0 breast cancer may have ductal carcinoma in situ or DCIS, in which abnormal cells have developed in the lining of a breast duct.

The omega - 3s were effective against all kinds of cells that were cancerous, but the effect was seen to be more pronounced in triple - negative breast cancer cell lines, with a proliferation reduction of up to 90 %.

Bottom line While research has shown some benefits with regards to ER + breast cancer cell death and prevention of metastases within mice and cellular models, it is recommended that human intake should be through diet only, not supplementation.