After binding, cells were washed once (carefully using a 200 μl pipette to remove the medium) with RPMI with 0.5 % BSA solution and adherent cells were analyzed by immunofluorescence microscopy or
bright field microscopy for quantification.
Not exact matches
The researchers noted that while their current work is focused on fluorescent applications, FlatScope could also be used for
bright -
field, dark -
field and reflected - light
microscopy.
«With this algorithm, we present a new way to achieve 3D quantitative phase
microscopy using a conventional
bright -
field microscope,» says Adrien Descloux, one of the lead authors of the paper.
We adapted a pseudo-speckle technique that can be applied to
bright -
field and fluorescent
microscopy time series.
Expression of colA1, which encodes collagen type A, first using traditional
bright -
field microscopy and again using WMISH.