Sentences with phrase «buffered saline»

All antibodies were diluted in phosphate buffered saline containing 0.05 % Tween (PBS - T).

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide, and freeze - dried.

Following electrophoresis, proteins were transferred to polyvinylidene difluoride membrane, and the membrane was blocked with 5.0 % nonfat dry milk in 0.1 % Tween - 20 in Tris buffered saline (TBS) for 1 h.

After receipt, the blood tubes were inspected to ensure anticoagulation and aliquots of blood were diluted 1:1 with phosphate buffered saline pH 8.0.

Mice were sacrificed between 5 days and 20 weeks post infection by transcardial perfusion with 20 mL ice - cold sterile phosphate buffered saline following anesthesia with 500 µL 2.5 % avertin administered intraperitoneally (i.p.).

Mononuclear cell pellets were separated from the diluted blood specimen by centrifugation with ficoll (400 g, 30 min) and the mononuclear cell layer was removed from the tube using a transfer pipette, resuspended in a phosphate buffered saline solution, and briefly centrifuged again.

The membranes were blocked in 5 % nonfat milk (NFM) protein in Tris - buffered saline containing 0.05 % Tween (TBS - T).

After electrophoresis, samples were electro - transferred to nitrocellulose PROTRAN membranes, blocked with 5 % dried skimmed milk in 50 mM Tris pH 8.4, 0.9 % NaCl, 0.05 % Tween 20 (Tris buffered saline - Tween 20, TBS - T), and incubated overnight in the presence of an anti-COX-2 (at a dilution of 1 ∶ 500) or anti-beta actin (at a dilution of 1 ∶ 3500) monoclonal antibodies (Santa Cruz Biotechnology and Sigma, respectively).

Samples for histology were collected as for microbial analysis (see above), with the same sample number of samples however, they were preserved in 5 % paraformaldehyde made up with Phosphate Buffer Saline (PBS).

↵ 3 The abbreviations used are: CDK, cyclin - dependent kinase; mAb, monoclonal antibody; TBS, Tris - buffered saline.

Sections were incubated in 0.3 % hydrogen peroxide in PBS for 20 min, washed in PBS - T (0.01 M phosphate - buffered saline, 0.2 % Triton X-100) and blocked 1 h with 10 % normal goat serum (NGS) in PBS - T.

Non-confluent cultures were trypsinized into single - cell suspension, counted, washed with phosphate - buffered saline (PBS), and fixed with 10 % paraformaldehyde.

Dissected wild - type or CCHa2 - R mutant brains were immersed in phosphate buffered saline (PBS).

At the end of testing at P100 or P150, rats were euthanized with sodium pentobarbital (Sigma) overdose and perfused with phosphate buffered saline (PBS).

For intracardiac injections, cells were harvested from subconfluent culture plates, washed with phosphate - buffered saline (PBS), and resuspended at 106 / ml (1833) or 5 × 106 / ml (SCP28) in PBS; 0.1 ml of the suspended cells was injected into the left cardiac ventricle using 30 - gauge needles.

Brain or ovary tissues from the affected ducks were homogenized in sterile phosphate - buffered saline (PBS, pH 7.2) to give a 20 % suspension (w / v).

The devices are aged in three solutions — phosphate ‐ buffered saline (PBS), 30 × 10 − 3 and 150 × 10 − 3m H2O2 / PBS — and stressed using cyclic voltammetry (2500 cycles) and 20 million biphasic pulses.

Cells were washed three times with ice - cold 1X PBS, scraped into 1 ml of phosphate buffered saline (PBS) containing protease inhibitors (1x G - Biosciences Protease Arrest, 200 µM Na3VO4, and 1 mM PMSF) and collected by centrifugation (700xg for 4 min).

Cells were washed in phosphate - buffered saline (PBS) containing calcium and magnesium, fixed in 4 % paraformaldehyde in PBS for 10 minutes, washed twice with PBS, and then permeabilized with 0.2 % Triton - X100 in PBS for 5 minutes.

The colonies were fixed in culture dishes with 4 % paraformaldehyde in phosphate - buffered saline (0.01 M PBS, pH 7.4) for 20 min at room temperature (RT) followed by washing with PBS (2 × 5 min).

Cells were fixed with methanol: acetone (1:1) at − 20 °C for 20 min and then washed three times with phosphate - buffered saline containing 3 % bovine serum albumin.

Cell pellets were resuspended in phosphate - buffered saline, total cell count was performed, and then cytospins were prepared with 5000 cells.

The plates were blocked with 2 % Marvel milk powder in phosphate buffered saline (PBS) for 2 h at 37 °C, washed three times with PBS, 0.05 % Tween 20.

Wing and leg discs were dissected from post-defecation prepupae under RNAse free conditions in 1X phosphate - buffered saline.

To detect the expression of p53, the HepG2 cells were seeded at 1 × 106 cells / well on microscope cover glasses in 6 - well plates overnight before being treated with the drugs for the appropriate time periods, followed by washing with phosphate - buffered saline and fixing with ethanol.

They were immediately delivered to the laboratory within 2 to 6 hours and washed thoroughly with sterile phosphate buffered saline (PBS) to remove excess blood cells.

The spectroscopic data obtained of purified protein in phosphate - buffered saline (PBS) are summarized in Table 1.

The primary antibody for p53 (see Table 1) was diluted 1:500 in phosphate buffered saline (PBS).

For that purpose, 21 - to 23 - month - old mice were treated with daily injections of either recombinant GDF11 (rGDF11, 0.1 mg / kg mouse body weight), a dosing regimen that increases GDF11 levels in old mice toward youthful levels (13), or phosphate - buffered saline (PBS)(vehicle) for 4 weeks, and their blood vessels were subsequently analyzed by using the volumetric assay described above.

The authors administered daily injections of GDF11 into old mice for 4 weeks, and found an increase in vasculature (by CD31 stain) and neurogenesis in the subventricular zone (by Sox2 stain), compared with control mice injected with phosphate buffered saline.

The rats were first treated with kainic acid and phosphate buffered saline (PBS), both of which are substances which scientists use to deliberately increase free radicals so they can then test treatments for oxidative stress.

I would suggest using cotton pads soaked with sterile buffered saline to gently clean his eyes at least twice daily.