Sentences with phrase «buffered saline containing»

All antibodies were diluted in phosphate buffered saline containing 0.05 % Tween (PBS - T).

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide, and freeze - dried.

The membranes were blocked in 5 % nonfat milk (NFM) protein in Tris - buffered saline containing 0.05 % Tween (TBS - T).

Not exact matches

Cells were washed three times with ice - cold 1X PBS, scraped into 1 ml of phosphate buffered saline (PBS) containing protease inhibitors (1x G - Biosciences Protease Arrest, 200 µM Na3VO4, and 1 mM PMSF) and collected by centrifugation (700xg for 4 min).

Cells were washed in phosphate - buffered saline (PBS) containing calcium and magnesium, fixed in 4 % paraformaldehyde in PBS for 10 minutes, washed twice with PBS, and then permeabilized with 0.2 % Triton - X100 in PBS for 5 minutes.

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.