Sentences with phrase «buffered saline following»
Mice were sacrificed between 5 days and 20 weeks post infection by transcardial perfusion with 20 mL ice - cold sterile phosphate buffered saline following anesthesia with 500 µL 2.5 % avertin administered intraperitoneally (i.p.).
http://journals.plos.org/ Not exact matches
The colonies were fixed in culture dishes with 4 % paraformaldehyde in phosphate - buffered saline (0.01 M PBS, pH 7.4) for 20 min at room temperature (RT) followed by washing with PBS (2 × 5 min).
To detect the expression of p53, the HepG2 cells were seeded at 1 × 106 cells / well on microscope cover glasses in 6 - well plates overnight before being treated with the drugs for the appropriate time periods, followed by washing with phosphate - buffered saline and fixing with ethanol.
At the end of the experiment, birds were killed by an overdose of Narcoren (Merial, Hallbergmoos, Germany) and transcardially perfused with 0,12 M phosphate buffered saline (PBS) followed by 4 % paraformaldehyde (PFA) dissolved in PBS.