Sentences with phrase «buffered saline ph»
The sections were submerged in antigen retrieval (citrate buffer saline pH 8.4) in an oven at 95 °C for 20 minutes and then left at room temperature for 20 minutes to cool.
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Brain or ovary tissues from the affected ducks were homogenized in sterile phosphate - buffered saline (PBS, pH 7.2) to give a 20 % suspension (w / v).
The colonies were fixed in culture dishes with 4 % paraformaldehyde in phosphate - buffered saline (0.01 M PBS, pH 7.4) for 20 min at room temperature (RT) followed by washing with PBS (2 × 5 min).
The lungs were washed twice, with 1 ml of phosphate - buffered saline, pH 7.4, and 2 ml of bronchoalveolar lavage (BAL) fluid was collected.