Direct immunofluorescence for presence of antigen from smears of conjunctival, tonsillar and genital epithelium, and
buffy coat.
Unlike the canine situation, it is not all that helpful to stage the disease with
buffy coat smears and bone marrow taps, although localizing the disease to the spleen with a splenic aspirate might be particularly useful as tumor cells in these locations have not altered prognosis.
But Wait, There's More to a PCV Than Red Blood Cells The remainder of the volume in the capillary tube is a few percentages of white blood cells and platelets in a section called
the buffy coat.
On arrival, the samples were centrifuged (1200 × g, 15 min, room temperature) to separate plasma from
buffy coat and red blood cells, and the samples were frozen in liquid nitrogen until they were analyzed.
FMc was identified and quantified in genomic DNA extracted from peripheral blood
buffy coat specimens employing quantitative PCR for a Y chromosome specific gene, DYS14.
To our knowledge, there are no studies of FMc in
buffy coat.
The key constituents more greatly represented in
buffy coat compared to Ficoll - purified cells are large granular cells such as neutrophils or dendritic cells.
Germline DNA was extracted from
buffy coat using the DNeasy Blood and Tissue Kit (QIAGEN; manufacturer's instructions) and quantified with a NanoDrop spectrophotometer (Thermo Scientific).
HAT sensitive derivative of CEM, a human T - cell line derived from the peripheral blood
buffy coat of a four - year old Caucasian female with acute lymphoblastic leaukemia.