Immunoblot analysis of recombinant antigens and B.
burgdorferi lysates with representative sera from mice inoculated by i.p. / s.c. routes with B31 - A3 WT, ospC7 mutant, and ospC7 / ospC +4 complemented B. burgdorferi clones.
Sera from mice obtained 3 weeks after tick - feeding were tested against recombinant B. burgdorferi antigens and whole - cell B.
burgdorferi lysates.
Not exact matches
Whole - cell
lysates from B.
burgdorferi and from Escherichia coli expressing recombinant P39 [BmpA (Borrelia membrane protein A)-RSB-(19) or recombinant His - tagged OspC (20) were prepared as described in ref.
Antibody titers against B.
burgdorferi cN40
lysates were determined by enzyme - linked immunosorbent assay (ELISA).