Sentences with phrase «by cre»

It also is protected by CRE ONLINE INC's copyright.

The suburban office park has been taking a beating lately, by both CRE experts and the media, who claim that this mid-20th-century phenomenon is dying, just like shopping malls.

This research study by the CRE's ROI committee examined how different approaches to four TV measures used in models affected granularity, precision and accuracy.

This design permits excision of all but 238 bp of inert proviral DNA, lacking both promoter activity and polyadenylation signals, following cleavage by Cre.

We have generated new genetic tools for the analysis of in vivo mast cell functions that allow for cell type - specific inactivation («knock out «-RRB- of target genes selectively in mast cells by Cre loxP - mediated recombination.

The selectable marker can be removed by Cre - mediated excision for scarless tag insertion.

Phenotypes induced by Cre driver, tamoxifen toxicity, Cre leakiness: What are the unexpected complexities and technical limitations?

In these mouse lines, gene knockout can be induced by Cre recombinase expression while proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP - FRT.

Impaired Ca2 + Signaling in β - Cells Lacking Leptin Receptors by Cre - loxP Recombination.

Site - specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1.

As early as 1998, the Cooperation Programme in Europe for Research on Nature and Industry through Coordinated University Studies (COPERNICUS) was launched by CRE, the predecessor of the European Universities Association (EUA).

Not exact matches

But this shift is happening within the overall downturn of CRE, after a most phenomenal seven - year price surge, funded by cheap loans that are now getting a lot more expensive.

Most CRE infections occur in hospitals and long - term care facilities among patients who are already weakened by unrelated disease or have undergone certain medical procedures.

Smith added that while these drugs might be used by themselves to treat CRE infection, they could also be used as starting points for further drug development.

Carbapenem - resistant Enterobacteriaceae (CRE), which include Klebsiella, were listed as one of the top three urgent antibiotic resistant threats in 2013 by the Centers for Disease Control and Prevention.

To take that step, researchers from the Max Planck Institute for Molecular Cell Biology and Genetics and the University of Hamburg's Heinrich Pette Institute for Experimental Virology and Immunology began with the bacterial enzyme Cre recombinase, which exchanges any two pieces of DNA flanked on either end by a certain pattern of nucleotides (DNA subunits) known as loxP.

CREs are regions of genome DNA that do not code for proteins, and control morphology and other traits by regulating genes.

Because the ASHCEs in genes such as Sim1 were highly conserved and therefore largely unchanged by evolution since the dinosaur era, this suggests CREs such as ASHCEs were vital in developing bird - specific traits and may have driven the transition of dinosaurs to birds.

The Gad2 - IRES - Cre knock - in allele was designed by Dr. Z. Josh Huang (Cold Spring Harbor Laboratory).

PHENOMIN aims to maximize the utility of this resource by offering the scientific community with the possibility to generate and characterize new Cre or CreERT2 lines.

Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus.

Together, these reporter gene experiments confirmed the expression of Tie2 by pericytes and further validated Ng2 - Cre as a robust pan-pericyte driver for in vivo mutagenesis studies.

Tie2PEKO were generated by breeding Ng2 - Cre mice (Jackson Laboratory, # 008533) 39 with Tie2fl / fl mice bred in the C57 / Bl6 background37.

By emulating nature's design principles, a team at Harvard's Wyss Institute for Biologically Inspired Engineering, Harvard Medical School and Dana - Farber Cancer Institute has cre... Read more

Ferguson BW, Gao X, Kil H, Lee J, Benavides F, Abba MC, Aldaz CM (2012) Conditional Wwox deletion in mouse mammary gland by means of two Cre recombinase approaches.

For example the researchers used the CRE - ERT2 mouse model validated at the ICS, in which Cre function is controlled by the promoter of Atypical Notch ligand Delta - like homologue1 (Dlk1) under Tamoxifen activation, a skin fibroblast marker, to carry out temporal and tissue specific inactivation of key genes involved in skin fibroblast diversiCRE - ERT2 mouse model validated at the ICS, in which Cre function is controlled by the promoter of Atypical Notch ligand Delta - like homologue1 (Dlk1) under Tamoxifen activation, a skin fibroblast marker, to carry out temporal and tissue specific inactivation of key genes involved in skin fibroblast diversiCre function is controlled by the promoter of Atypical Notch ligand Delta - like homologue1 (Dlk1) under Tamoxifen activation, a skin fibroblast marker, to carry out temporal and tissue specific inactivation of key genes involved in skin fibroblast diversity.

In addition to improving the Brainbow system, Sanes and Lichtman also developed a complementary Flpbow construct that is functionally similar to Cre - based Brainbow, but is controlled by FLP / FRT recombinase.

al were able to create a split version of Cre that is only active when dimerized by the GBPs / GFP.

These sites are recognized by an enzyme called Cre recombinase, which binds to them and inverts the genetic sequence between them.

The new system, CRE - DOG (Cre Dependent On GFP), is activated by GFP and derivatives GFP and YFP, but not red fluorescent proteins, as seen with T - DDOCRE - DOG (Cre Dependent On GFP), is activated by GFP and derivatives GFP and YFP, but not red fluorescent proteins, as seen with T - DDOCre Dependent On GFP), is activated by GFP and derivatives GFP and YFP, but not red fluorescent proteins, as seen with T - DDOGs.

The progranulin - insufficient mice used for this study were bred from a line generated by crossing Grn - floxed mice with mice carrying β - actin Cre, which were subsequently back - crossed onto a C57BL / 6J background (Martens et al., 2012; Filiano et al., 2013).

were able to create a split version of Cre that is only active when dimerized by the GBPs / GFP.

By injecting the modified viruses into transgenic mice that only express Cre recombinase in astrocytes, the researchers knew that any sequences flagged by the lox site inversion had successfully transferred their genetic cargo to the target cell type — here, astrocyteBy injecting the modified viruses into transgenic mice that only express Cre recombinase in astrocytes, the researchers knew that any sequences flagged by the lox site inversion had successfully transferred their genetic cargo to the target cell type — here, astrocyteby the lox site inversion had successfully transferred their genetic cargo to the target cell type — here, astrocytes.

In some cases affected transcripts in Cre +; Tsc1 flox / flox mice showed significant variation among individuals that can not simply be explained by differential effectiveness of CRE - mediated knockout since, except one animal, all Cre +; Tsc1 flox / flox mice showed similarly reduced expression levels of Tsc1 mRCre +; Tsc1 flox / flox mice showed significant variation among individuals that can not simply be explained by differential effectiveness of CRE - mediated knockout since, except one animal, all Cre +; Tsc1 flox / flox mice showed similarly reduced expression levels of Tsc1 mRCRE - mediated knockout since, except one animal, all Cre +; Tsc1 flox / flox mice showed similarly reduced expression levels of Tsc1 mRCre +; Tsc1 flox / flox mice showed similarly reduced expression levels of Tsc1 mRNA.

In a second approach, [3H] retinol - radiolabeled ApoC - III — depleted and ApoC - III — bearing TRLs (Figure 9A and Supplemental Figure 6) were evaluated for their uptake by primary hepatocytes isolated from Ndst1fl / fl Alb - Cre + and Ldlr — / — Lrp1fl / fl Alb - Cre + mice.

These results support the notion that the increased clearance of ApoC - III — depleted [3H] TRLs in Ndst1fl / fl Alb - Cre + mice, and not in Ldlr — / — Lrp1fl / fl Alb - Cre + mice, is a consequence of increased uptake by LDLR / LRP1 on hepatocytes.

ApoC - III ASO treatment reduced TG levels in chylomicrons, VLDL, and remnant particles in all of the mutants as measured by size - exclusion fast protein liquid chromatography (FPLC)(Figure 2, A — E), but had no effect in Ldlr — / — Lrp1fl / fl Alb - Cre + mice (Figure 2F).

In conjunction with these strains, several cre transgenic strains have been created where BAC engineering was used to insert an intron containing cre or creERT2 cassettes, followed by a polyadenylation sequence to terminate transcription of the fusion transcript immediately after the recombinase gene, into the BAC vector at the initiating ATG codon in the first coding exon of the gene.

To verify that the cortical LPA signals associated with non-HEV structures were derived from FRCs, we next mapped the LPA signals relative to HEVs in Enpp2fl / fl mice and Ccl19 - Cre Enpp2fl / fl mice by measuring the distance between individual signals and the nearest HEV.

Intranodal T - cell migration in Enpp2fl / fl mice and Ccl19 - Cre Enpp2fl / fl mice, analyzed by intravital two - photon microscopy.

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There is very little evidence that the CRE curriculum supports intellectual quality of learning recommended by the DEECD framework of what constitutes high quality teaching and learning practice in the classroom.

In all likelihood, if you were to embellish your income on a cre dit application by a few thousand dollars, it may go unnoticed.

Search our member database for CRE's who service your region or live nearby, as well as by specialty.

Before moving to CRES, Dr. Pigott was employed by a private practice referral hospital in Wisconsin where he worked in both elective and emergency orthopedic and soft tissue surgery, advanced imaging and lameness.

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The meteorites are dated by analysing isotopic changes in their matter due to cosmic ray exposure (CRE dating [Eugster, 2003]-RRB-.

For the reasons detailed below, the Center for Regulatory Effectiveness («CRE») respectfully requests that you withdraw dissemination of the first National Assessment on Climate Change («National Assessment»)(http://www.usgcrp.gov/usgcrp/nacc/default.htm), Withdrawal of the National Assessment is necessary because: (1) there are numerous data quality and scientific flaws in it; and (2) it does not comply with the Federal Data Quality Act («FDQA»), 44 U.S.C. § 3516 note, and with guidelines promulgated by the Office of Management and Budget («OMB») implementing the FDQA, 67 Fed.

The cloud radiative effect (CRE) on the Earth's present - day radiation budget can be inferred from satellite data by comparing upwelling radiation in cloudy and non-cloudy regions.

«It should also be noted that Müller and Grooß87, 88 recently criticized the CRE and CFC - warming theories by presenting the so - called «ACE - FTS satellite data».