TFUMseq (Temporal FUnctional Metagenomics sequencing) uses shotgun libraries cloned into a recipient bacterial species, tracked over time in
gnotobiotic mice
by deep sequencing and computational methods.
A service to derive germ - free (axenic) mice is now provided
by three infrastructures with a long standing expertise and track record in the derivation of axenic and
gnotobiotic mice, namely the Gnoto / Axenic Facility of the Instituto Gulbenkian de Ciência, the Karolinska Institutet Core Facility for Germ - free Research, and the CNRS Service Isotechnie of the PHENOMIN - TAAM infrastructure.