Sentences with phrase «by agarose»

The SLC6A4 promoter VNTR polymorphism was genotyped by agarose gel size fractionation as described in ref.

The resulting PCR products were separated by agarose gel electrophoresis.

Digested DNA was visualized by agarose gel (1.5 — 2.0 %).

Ten - microliter aliquots of the nested PCR products were subjected to RFLP analysis by digestion with 2 U of either HinfI or MseI, and digested fragments were resolved by agarose gel electrophoresis in TBE buffer, as described elsewhere [19, 28].

The resulting DNA fragments are analyzed by agarose gel electrophoresis.

Not exact matches

He and his colleagues wanted to avoid the classic light - sheet arrangement in which the sample is embedded in a tube of agarose and surrounded by lasers and cameras.

Amplified sequences were visualized by gel electrophoresis in 2 % agarose gels stained with GelRed (Biotium).

PCR products were separated by electrophoresis on a 2 % agarose gel, stained with ethidium bromide and visualized by UV illumination.

PCR products were visualized by electrophoresis in an ethidium bromide - stained 1.8 % agarose gel.

After a traditional PCR has been completed, the data are analyzed by resolution through an agarose gel or, more recently, through a capillary electrophoresis system.

DNA libraries were then re-amplified for another 13 cycles in quintuplicates or sextuplicates, followed by pooling and purification, visual inspection on a 3.5 % agarose gel, and final quantification using a NanoDrop 2000c spectrophotometer (FisherScientific).

PCR products were separated by gel electrophoresis on 2 % agarose gel.

The resulting PCR amplification products were analyzed by electrophoresis in 1.5 % agarose gels.

To generate a full - length unc - 9:: gfp construct, the 14.7 - kb UNC - 9A + B PCR product (representing the 5» end of unc - 9) and the unc - 9:: gfp plasmid were digested separately with Kpn I, digests electrophoresed through low - melt agarose (SeaPlaque GTG agarose, FMC, Rockland, ME, USA), and appropriate fragments purified by phenol extraction and ethanol precipitation.

At 24 hours post fertilization (hpf), fluorescent embryos were dechorionated, mounted on coverslips in 1.2 % ultra-low gelling agarose, overlaid with 30 % Danieua / PTU and analyzed by laser scanning confocal microscopy (Zeiss LSM510 Meta).

PCR products were visualized by blue light after electrophoresis on a 2.5 % agarose gel containing 1 × GelStar ® Nucleic Acid Gel Stain (Lonza, Breda, The Netherlands) in 1 × Tris - borate buffer (pH 8.0).

The supernatant was mixed with 300 µl of Ni - NTA agarose (Qiagen), and the proteins were allowed to bind for 3 hours, followed by washing with 20 ml of resuspension buffer, and eluted with 500 µl of elution buffer (20 mM NaHPO4, 300 mM NaCl, 10 % glycerol, 150 mM Imidazole, pH 7.8).