Sentences with phrase «by agarose gel»
The SLC6A4 promoter VNTR polymorphism was genotyped by agarose gel size fractionation as described in ref.
http://www.pnas.org/
The resulting PCR products were separated by agarose gel electrophoresis.
Digested DNA was visualized by agarose gel (1.5 — 2.0 %).
Ten - microliter aliquots of the nested PCR products were subjected to RFLP analysis by digestion with 2 U of either HinfI or MseI, and digested fragments were resolved by agarose gel electrophoresis in TBE buffer, as described elsewhere [19, 28].
The resulting DNA fragments are analyzed by agarose gel electrophoresis.
Not exact matches
Amplified sequences were visualized by gel electrophoresis in 2 % agarose gels stained with GelRed (Biotium).
PCR products were separated by electrophoresis on a 2 % agarose gel, stained with ethidium bromide and visualized by UV illumination.
PCR products were visualized by electrophoresis in an ethidium bromide - stained 1.8 % agarose gel.
After a traditional PCR has been completed, the data are analyzed by resolution through an agarose gel or, more recently, through a capillary electrophoresis system.
DNA libraries were then re-amplified for another 13 cycles in quintuplicates or sextuplicates, followed by pooling and purification, visual inspection on a 3.5 % agarose gel, and final quantification using a NanoDrop 2000c spectrophotometer (FisherScientific).
PCR products were separated by gel electrophoresis on 2 % agarose gel.
The resulting PCR amplification products were analyzed by electrophoresis in 1.5 % agarose gels.
At 24 hours post fertilization (hpf), fluorescent embryos were dechorionated, mounted on coverslips in 1.2 % ultra-low gelling agarose, overlaid with 30 % Danieua / PTU and analyzed by laser scanning confocal microscopy (Zeiss LSM510 Meta).
PCR products were visualized by blue light after electrophoresis on a 2.5 % agarose gel containing 1 × GelStar ® Nucleic Acid Gel Stain (Lonza, Breda, The Netherlands) in 1 × Tris - borate buffer (pH 8.gel containing 1 × GelStar ® Nucleic Acid Gel Stain (Lonza, Breda, The Netherlands) in 1 × Tris - borate buffer (pH 8.Gel Stain (Lonza, Breda, The Netherlands) in 1 × Tris - borate buffer (pH 8.0).