In cases of neonatal mortality, the diagnosis typically is made postmortem with virus isolation from fresh lung, liver, kidney, and spleen
by cell culture techniques and subsequent identification by PCR and sequencing, transmission electron microscopy, immunofluorescence, or fluorescence in situ hybridization.
Not exact matches
She was always ready to learn new
techniques, and she diversified her skill set
by working in the animal facility,
cell culture room, and also in a mass spectrometry lab.
He improved the
technique by culturing the stem
cells with astrocytes,
cells that guide the maturing neurons, that he had taken from the dopamine - producing region of the rat brain.
Virus isolation is usually achieved using in vitro
cell culture techniques, but this is not easily achieved from CCD bees because many are infected
by multiple viruses and plaque purification
techniques are not available for most of these viruses.
This unique workshop provides the introduction and practical hands - on of integrated metabolomics workflows, employing the
techniques of cellular energy metabolism (Agilent Seahorse XF), detailed metabolomic analysis
by LC / MS Q - TOF and the translation of
cell culture results into a living mouse model using indirect calorimetry.
Finally, we have developed new
techniques utilizing surface sensing of translation (SUnSET) and bioorthogonal noncanonical amino acid tagging (BONCAT) coupled with stable isotope labeling
by amino acid in
cell culture (SILAC) to selectively isolate and quantify newly synthesized proteins within specific temporal windows.