Sentences with phrase «by centrifugation»

Spheroids were resuspended in 1 ml EC basal medium and collected by centrifugation at 200g for 5 min.

Beads were collected by centrifugation at 4000 RPMs for 5 minutes.

Virus was concentrated by centrifugation at 90,000 x g for 2 hours at 4 °C, and resuspended in 200 µl PBS.

Mononuclear cell pellets were separated from the diluted blood specimen by centrifugation with ficoll (400 g, 30 min) and the mononuclear cell layer was removed from the tube using a transfer pipette, resuspended in a phosphate buffered saline solution, and briefly centrifuged again.

Pellet the cultured cells by centrifugation for 5 minutes at 1000 x g (approximately 2000 rpm) at 4 °C.

Bacteria were allowed to express the recombinant protein overnight at room temperature, followed by harvesting by centrifugation at 6000 rpm for 10 min.

DNA was pelleted by centrifugation at max speed for 30 minutes at 4 °C.

FBS (Sigma) was added to the tissue followed by centrifugation at 1000 x g for 5 min at 4 °C.

Plasma was recovered from the EDTA - treated blood by centrifugation at 15,000 × g for 20 minutes and aliquoted and frozen at -80 °C until use.

In order to remove such highly toxic solvents, complicated processes are necessary, such as washing with water, separation by centrifugation, compression, etc..

The bottom row shows the grams of nanoparticles after being purified by centrifugation.

The researchers made a thin film of graphene oxide by chemically exfoliating graphite into graphene flakes, which were then mixed with water and concentrated by centrifugation into a thick slurry.

Clinical samples were also sent to an outside laboratory (Focus Diagnostics, Cypress, CA) for respiratory viral testing by centrifugation - enhanced shell vial culture followed by direct fluorescent antibody staining for 8 viruses (respiratory syncytial virus, adenovirus, influenza virus A and B, parainfluenza virus types 1, 2, and 3, and human metapneumovirus).

Clinical samples were clarified by centrifugation for 10 min × 4000 g and passaged through a 0.2 - µm filter.

Homogenates were cleared by centrifugation at 100.000 g for 1 h at 4 °C, and supernatants were collected for further analysis (TBS - soluble fraction).

Bone marrow - derived mesenchymal stem cells (BM - MSCs) are obtained by isolating mononucleated cells from a marrow aspirate by centrifugation on a density gradient, followed by adherent cell expansion in tissue culture.

Cell debris was removed from culture supernatant by centrifugation at 12,500 g for 45 min at 4 °C, and the virus particles were concentrated by ultracentrifugation at 45,000 r.p.m in a BECKMAN 70Ti rotor for 3 hrs.

PBMCs and plasma were immediately isolated by centrifugation of the CPT tubes.

Cell debris was removed from the sample by centrifugation at 13,000 rpm for 30 min at 4 °C and the supernatant diluted in sample buffer.

Your pet s fecal sample will be tested by a what is called a «fecal float» or «fecal evaluation by centrifugation».

Conduct fecal examinations by centrifugation at least four times through the first 12 months of life, and a minimum of two instances per year in adults, depending on affected person well being and way of life elements.

This includes the Lab 4Dx Plus Test, the Lyme Quant C6 Test to measure active Lyme infection, the best available fecal flotation by centrifugation, and new breakthrough fecal antigen ELISAs for hookworm, roundworm, and whipworm detection.

After each wash, beads were collected by centrifugation at 4000 RPMs for 5 minutes and supernatant was discarded.

Cell supernatants were harvested by centrifugation at 1,500 rpm for 5 minutes and then used for the measurement of IL - 22.

Nuclei were pelleted by centrifugation (5000 rpm for 5 min) and resuspended in 350 µl nuclear lysis buffer [50 mM Tris pH 8.1, 10 mM EDTA, 1 % SDS] containing protease inhibitors.

The mixture was sonicated on ice with 0.1 % Triton X-100 for 30 min, followed by centrifugation at 13,000 rpm for 30 min.

Trypsin was inactivated with an equal volume of FBS containing medium and the cells were collected by centrifugation at 1000 x g for 10 minutes.

Cells were washed three times with ice - cold 1X PBS, scraped into 1 ml of phosphate buffered saline (PBS) containing protease inhibitors (1x G - Biosciences Protease Arrest, 200 µM Na3VO4, and 1 mM PMSF) and collected by centrifugation (700xg for 4 min).

In other cases the process can be speeded up by centrifugation.