The adherent blood cells over their luminal surface were visualized by scanning electron microscopy (SEM) and
by confocal microscopy after immunostaining with mouse anti-CD41 antibody tagged with PE, rabbit anti-fibronectin and mouse anti-β − actin.
Immunocytochemical staining for Ki67 (cell proliferation marker), Sox2 (neural stem cell marker), Olig2 (progenitor cell marker), and GFAP (glial fibrillary acidic protein, a marker for differentiated astrocytes) captured
by confocal microscopy imaging.
For immunohistochemical analyses, serial sections were stained with Alexa Fluor 594 - conjugated MECA - 79 and Hoechst 33342, and
examined by confocal microscopy (LSM710; Zeiss, Germany).
Studies have shown that corneal nerve fiber density,
measured by confocal microscopy, can predict diabetes - related damage to the peripheral nerves, called peripheral neuropathy.