Sentences with phrase «by culturing cells»

Oligodendrocyte differentiation was induced by culturing the cells in DMEM / F12, 1 % N2, and IGF - 1 (200 ng / ml).

By culturing cells in a fed - batch system with StemSpan ™ medium containing cytokines and UM171, expanded cells were found to successfully engraft and repopulate immunocompromised mice, with no disadvantage when compared to unmanipulated cells.

Producing «clean» meat by culturing cells — instead of raising or slaughtering animals - is a new frontier in food production that will require consumer education and transparent labeling.

Producing «clean» meat by culturing cells — instead of raising or slaughtering animals - is a new frontier in food production, but will it require a new regulatory framework, and what should we call it?

Bay area start - up Finless Foods — one of a new wave of cellular agriculture companies producing meat by culturing cells (without raising or slaughtering animals)-- has slashed production costs by 50 % since September and is aiming to achieve price parity...

Not exact matches

Rather than obtaining meat from animals raised on environmentally destructive factory farms and slaughtered in filthy slaughterhouses, clean meat is produced by taking a small sample of animal cells and replicating them in a culture outside of the animal.

I was inspired by the vulnerability of that culture; the way people spill their life out over their cell phones while you're sitting next to them on the subway.

[Perhaps the concept of «church - court» has moved to the openness of the Internet, and we all are now part of the greater «church court»... but then again, I can only wonder how to implement the third step within a global culture interconnected by the Internet, cell phones, tweets, and videos....

In both cases individual members may exercise some dominance over others, in particular by altering the patterns guiding further growth and development, but the social coordination stems from basic patterns embodied in the genetic makeup of the plant cells and in the laws and traditions of human culture.

... Researchers at Penn State University have found that three water soluble chemicals in garlic reduced cholesterol production in cultured rat liver cells by 40 - 60 percent.

While some still object to cells being taken from animals and used by scientists to grow clean meat in laboratories — and some just don't like the idea of eating a «cultured» steak created by men and women in white coats — others see the lab - grown meat revolution as key to solving the environmental crisis linked to meat eating.

At the beginning of 2011 we launched our first ingredient fully produced by plant cell culture: Resistem, an anti-ageing ingredient.

Rather than obtaining meat from animals raised on environmentally destructive factory farms and slaughtered in filthy slaughterhouses, clean meat is produced by taking a small sample of animal cells and replicating them in a culture outside of the animal.

The researchers experimented with inducing oxidative stress in a human cell line culture with and without VCOP (virgin coconut oil polyphenols) to observe how VCOP positively promoted catalase, a very important enzyme in protecting the cell from oxidative damage, and glutathione (GSH), a self - recycling antioxidant produced by the liver.

EGFR / WT and KO cells were cultured overnight and starved for 24 h. Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -cells were cultured overnight and starved for 24 h. Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -Cells were lysed and cox - 2 mRNA levels were analyzed by RT - PCR.

Visually, she is filming and analyzing time - lapse images of human embryos in the incubator and has been able to correlate various parameters of how cells divide with the probability that the embryos will make it to a full blastocyst stage by day 5 - 6 of culture.

She was always ready to learn new techniques, and she diversified her skill set by working in the animal facility, cell culture room, and also in a mass spectrometry lab.

Culturing stem cells by day, and audiences in dingy stand - up clubs by night, it wasn't until Helen Pilcher left the lab that she started basing her comedy on science.

The most promising chemical — sulforaphane, a naturally occurring compound found in cruciferous vegetables — tamped down glucose production by liver cells growing in culture, and shifted liver gene expression away from a diseased state in diabetic rats.

Researchers then tested cell cultures and mouse models by using a gene editing process called CRISPR - Cas9 to demonstrate how the presence or absence of myomaker and myomerger — both individually and in unison — affect cell fusion and muscle formation.

Using human fetal «mini-brains» grown in 3 - D cultures, scientists determined that a specific protein produced by the Zika virus changes the properties of neural stem cells in the developing brain of an infected fetus, potentially causing microcephaly in newborns (Ki - Jun Yoon, abstract 103.06, see attached summary).

A team spearheaded by Dr. Markus Helfer and Prof. Dr. Ruth Brack - Werner from the Institute of Virology and Prof. Dr. Philippe Schmitt - Kopplin from the Analytical BioGeoChemistry research unit (BGC) performed a detailed investigation of the effects of PS extracts on HIV - 1 infection of cultured cells.

Potti and his colleagues began by testing chemotherapy drugs on cultured cell lines from human tumors, such as from the lung, breast, or ovary.

Since the algae reproduces by cell division about once per day in the laboratory, numerous genetically identical cultures could be derived from the isolate.

Some of the leading non-animal tests are conducted on cell cultures, human and animal corneas from eye banks, corneal tissue cultures, and frozen corneas supplied by hospitals.

One would culture thin sheets of meat, seeded by cells from a living animal, on a reusable polymer scaffold; the other would grow meat on small edible beads that stretch with changes in temperature.

Subsequent tests revealed that the treatment had reduced the visible cell population by a factor of 100 thousand, compared with an untreated cell culture, within the space of just 20 days.

Although such harvested cells could be cultured as say, liver cells for treating hepatitis or dopamine - producing cells for Parkinson's, the resulting transplants would likely be rejected by patients» immune systems.

By studying the same process in cultured cells, the scientists found that the light stimulated a molecular chain reaction that activated a normally dormant protein called TGF - beta 1.

In cell culture, high expression levels of GPR161 induced proliferation of mammary epithelial cells, disrupted the acinar structures formed by these cells, and enhanced their invasive capacity.

By analyzing chemical changes of the IRS - 2 protein in immortalized cultures of human white blood cells, it determined that IRS - 2 appeared in two different forms — «on,» which allows the signal to pass through, and «off,» which stops the signal from activating the cells into M2 macrophages.

In experiments on cell cultures, both of these inhibitors succeeded in breaking various forms of the TKI resistance: including forms caused by additional mutations of the gene Bcr - Abl as well as those caused by large quantities of the protein Gab2.

As it can take weeks to grow human cells into intact differentiated and functional tissues within Organ Chips, such as those that mimic the lung and intestine, and researchers seek to understand how drugs, toxins or other perturbations alter tissue structure and function, the team at the Wyss Institute for Biologically Inspired Engineering led by Donald Ingber has been searching for ways to non-invasively monitor the health and maturity of cells cultured within these microfluidic devices over extended times.

A prototype device built by nScrypt, a company in Orlando, Florida, deposits one - hundredth - of - an - inch - thick dollops of lab - cultured cells onto a layer of gel - based paper invented at the University of Utah.

In 2011, researchers led by David Baker, a computational biologist at the University of Washington in Seattle, created a designer protein that binds HA's stem, which prevented viral infection in cell cultures.

When cells that produced IL - 21 were put in culture with synovial fibroblasts (which are the main contributors to joint inflammation in rheumatoid arthritis), they induced the production of proinflammatory cytokines by these synovial fibroblasts, and cells that do not produce IL - 21, did not demonstrate this same outcome.

The cells were put into a special culture and then suspended in a gel and stimulated by nutrients, all geared to turn them into neurons like those found in the cortex.

Mackay - Sim's team has solved those problems by developing a method of removing the cells under a local anaesthetic and growing them in culture to produce larger numbers.

Until recently, Ain was renowned for a highly prized repository of 18 immortal cancer cell lines, which he developed by harvesting tissue from his patients» tumors after removal, carefully culturing them to everlasting life in vials.

Currently, most influenza vaccines in the United States are produced using chicken eggs, while a few are made in cell culture or by using recombinant DNA technologies.

«By the 30th day of culture, there were obvious clumps of fluorescent cells visible under the microscope,» says lead author Valentin Sluch, Ph.D., a former Johns Hopkins biochemistry, cellular and molecular biology student and now a postdoctoral scholar working at Novartis, a pharmaceutical company.

In Europe and the United States, all kinds of cell preparations that are administered to patients following ex vivo culturing are classified as medicines, and monitored by drug agencies such as the US Food and Drug Administration, the European Medicines Agency and, in Italy, the Agenzia Italiana del Farmaco or AIFA.

They also suggest it may soon be possible to mature the cells in a laboratory dish simply by adding a cocktail of microRNAs to the cell culture.

Zheng, together with Leah Boyer, then a researcher in Gage's lab and now director of Salk's Stem Cell Core, generated diseased neurons by taking skin cells from patients with Leigh syndrome, reprogramming them into stem cells in culture and then coaxing them to develop into brain cells in a dish.

Despite a lack of rigorous clinical trials to test safety or efficacy, a ban of the treatment by health authorities, and a lack of peer review by the scientific community, the privately funded Stamina Foundation has been using cultured mesenchymal stem cells that have been exposed to putative conditions that favour neuronal differentiation to treat different diseases in severely or terminally ill patients.

A human brain cell culture grown in vitro has spontaneously transformed, as determined by morphology, growth characteristics, and karyotype analysis.

They showed that rapamycin reduced the secretion of inflammatory cytokines from senescent cells in culture and in mice by suppressing the mTOR pathway, which promotes growth.

The paper is titled, «Microenvironmental reprogramming by three - dimensional culture enables dermal papilla cells to induce de novo human hair follicle growth.»

The researchers found that fibroblasts and Hofbauer cells were susceptible to infection by Zika virus in isolated cultures.

The latter can be primed in tissue culture by providing small numbers of dendritic cells.