Sentences with phrase «by ectopic expression»

Genetic reprogramming to a pluripotent state of mouse somatic cells was first achieved by ectopic expression of four factors (Oct4, Sox2, Klf4 and c - Myc) using retroviruses [1].

Additionally, Western blot analysis indicated that the expression level of p21waf1 / cip1 gene was also enhanced by the ectopic expression of DDX3 in HuH - 7, HeLa, and 293T cells (Fig. 2B).

As shown in Fig. 4B, suppressive ability of miR - 7 on both transmigration through endothelial cells and Matrigel was significantly attenuated by ectopic expression of KLF4, suggesting that miR - 7 suppresses invasive ability of CSCs by blocking KLF4 expression.

Not exact matches

Subsequent procedures included mammalian somatic cell nuclear transfer, cell fusion, induction of pluripotency by ectopic gene expression, and direct reprogramming.

Dr. Guo says: «Muffs and beard in chicken is caused by a structural variation that consists of three duplicated regions on GGA27 and results in the ectopic expression of HOXB8 in facial skin.

Next, because KLF4 is involved in self - renewal of stem cell, we also examined the effect of both miR - 7 and KLF4 on proliferation of CSCs by MTS assay and found that miR - 7 was capable of blocking the growth of CSCs, whereas ectopic expression of KLF4 significantly mitigated this effect of miR - 7 (Fig. 4C).

Title: The developmental dismantling of pluripotency is reversed by ectopic Oct4 expression Authors: Osorno R, Tsakiridis A, Wong F, Cambray N, Economou C, Wilkie R, Blin G, Scotting PJ, Chambers I, Wilson V Date: 2012 Publication Details: Development 2012 139 (13): 2288 - 2298