Sentences with phrase «by electrophoresis»

The resulting PCR amplification products were analyzed by electrophoresis in 1.5 % agarose gels.

PCR products were visualized by electrophoresis in an ethidium bromide - stained 1.8 % agarose gel.

PCR products were separated by electrophoresis on a 2 % agarose gel, stained with ethidium bromide and visualized by UV illumination.

The success of the PCR amplification was confirmed by electrophoresis and 5 uL of post-PCR reaction product was treated to degrade unused primers and nucleotides using USB ® HT ExoSAP - IT ® High - Throughput PCR Product Cleanup (Affymetrix, http://www.affymetrix.com) according to the manufacturer's instructions.

Not exact matches

The appearance of the variant after starch - gel electrophoresis of the hemolysates supports the concept that the lactic dehydrogenase isozymes are determined by the interaction of two subunits which are under separate genetic control.

By combining two chemical techniques — narrow capillary electrophoresis and mass spectrometry — Moini figured out how to measure that ratio in just 20 minutes using microscopic samples of silk.

The ultimate goal is to put not just electrophoresis, but the preparation of DNA and the analysis of the DNA patterns all onto a single device controlled by a built - in computer.

Our new electrophoresis method allows us to increase the number of test samples on a given run by up to ten-fold and to complete the assay in less than half the time of the traditional comet process.»

Amplified sequences were visualized by gel electrophoresis in 2 % agarose gels stained with GelRed (Biotium).

Primers were chosen from exons separated by large introns, when possible, and PCR reaction quality and specificity were verified by gel electrophoresis and melting - curve dissociation analysis of the amplified product.

The 2 alleles differ by a single nucleotide, so restriction digestion of the amplified product followed by gel electrophoresis effectively differentiates the 2 alleles.

Clark's new techniques using capillary electrophoresis and microchip technology may allow for real - time field screening of plutonium and other materials by nuclear proliferation investigators and forensic scientists at the Department of Homeland Security.

The resulting DNA fragments are analyzed by agarose gel electrophoresis.

Indian scientists have developed a fluorescence - based system that can reveal a whole load of information about proteins separated by gel electrophoresis while they're still embedded in the gel.

Have basic equipment and skills to collect samples, extract and amplify DNA by PCR, and confirm by gel electrophoresis.

Ten - microliter aliquots of the nested PCR products were subjected to RFLP analysis by digestion with 2 U of either HinfI or MseI, and digested fragments were resolved by agarose gel electrophoresis in TBE buffer, as described elsewhere [19, 28].

Equal amounts of protein or conditioned medium were separated by SDS — polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes and probed with the indicated antibodies.

We offer a comprehensive selection of PCR - based B - and T - cell clonality assays for gel and capillary electrophoresis detection, which also include BIOMED - 2 reagents for clonality and translocations, exclusively offered by Invivoscribe.

Using Surveyor nuclease followed by gel electrophoresis, these mismatches are indicated by the presence of DNA cleavage products.

After a traditional PCR has been completed, the data are analyzed by resolution through an agarose gel or, more recently, through a capillary electrophoresis system.

The resulting PCR products were separated by agarose gel electrophoresis.

PCR products were separated by gel electrophoresis on 2 % agarose gel.

The size of the FLT3 ITD and NPM1 PCR products are determined by capillary electrophoresis.

Primer specificity was assessed by melting temperature analysis, gel electrophoresis and direct sequencing (Iowa State DNA Facility, Ames, IA).

PCR products were visualized by blue light after electrophoresis on a 2.5 % agarose gel containing 1 × GelStar ® Nucleic Acid Gel Stain (Lonza, Breda, The Netherlands) in 1 × Tris - borate buffer (pH 8.0).

Amplification products were analyzed by gel electrophoresis.

Nondenaturing 2 - dimensional gradient gel electrophoresis followed by apoA - I immunoblotting was performed on plasma from the patient and 17 control participants.20

Determination of D - saccharic acid -1,4-lactone from brewed kombucha broth by high - performance capillary electrophoresis.

Carrier screening for beta thalassemia and related hemoglobinopathies is recommended by ACOG via complete blood count and hemoglobin electrophoresis for people of African, Southeast Asian, Mediterranean, Middle Eastern, and West Indian descent considering having children.

Carrier screening for hemoglobinopathies such as sickle cell anemia is recommended by the American Congress of Obstetricians and Gynecologists (ACOG) via complete blood count and hemoglobin electrophoresis for people of African, Southeast Asian, Mediterranean, Middle Eastern, and West Indian descent considering having children.

It has a 6 inch screen with a resolution of 800 × 600 and is powered by a TFT screen, which uses electrophoresis without backlight, similar to the new ASUS Eee Tablet.

Other uses of electrophoresis include DNA testing, where electricity is used to separate the parts of a DNA sample by making them move across a gel, which enables them to be compared with other samples and identified.

The science behind epaper is called electrophoresis, and an electrophoretic display is basically the rearranging of charged pigment particles by applying a small electric field to them.

If some dogs have a high total protein evidence or evidence of an increased globulin fraction (globulin is one of the two types of serum proteins, the other being albumin) on a biochemistry profile, serum proteins should be examined by serum electrophoresis.

• Achieved success in providing closure to an extremely complex blood test by employing insight and relevant electrophoresis knowledge