Sentences with phrase «by embryo culture»

Not exact matches

Visually, she is filming and analyzing time - lapse images of human embryos in the incubator and has been able to correlate various parameters of how cells divide with the probability that the embryos will make it to a full blastocyst stage by day 5 - 6 of culture.

Additional studies evaluating the mechanisms by which race / ethnicity, infertility diagnosis, and day of embryo culture affect perinatal outcomes in both autologous [donor and recipient are the same person] and donor IVF pregnancies are warranted to develop preventive measures to increase the likelihood of obtaining a good perinatal outcome among ART users,» the authors write.

Although a clause in the law that funds NIH prevents the agency from funding research that would harm or destroy an embryo, a lawyer at the Department of Health and Human Services ruled in 1999 that because stem cells — which can grow ad infinitum in culture — are not themselves embryos, the NIH could fund work with cells that were derived by privately funded researchers or researchers overseas.

Previous experiments from other labs have successfully brought embryos more than 10 days into development by implanting them on a three - dimensional scaffold of endometrial cells and nutrients in culture.

The researchers at Penn and their collaborators used the Early Embryo Viability Assessment imaging device (or Eeva, developed and manufactured by Auxogyn, Inc.), which records images of developing embryos during the first three days of laboratory culture, to evaluate embryos transferred into the uterine cavity of 177 patients.

The growth potential of excised embryos cultured on 1/2 MS medium declined by 31 % after 1 month of dry storage or after 24 h of dry storage at − 18 °C (Table 3), but growth was similar to that of embryos from freshly collected seeds (c. 80 %) when embryos were cultured with growth hormones.

Embryo cultures were maintained at 22 — 24 °C for 8 weeks, in darkness for the first 2 weeks followed by a 16 - h / 8 - h light — dark cycle.

If Obama really wanted to resolve one front of the culture wars and show respect for pro-lifers, as he claimed, he would have refused to make citizens complicit in embryo killing by simply continuing the Bush policies.

This must be accomplished by using a process known as germ - line transmission, where the primordial germ cells (PGCs) of an early embryo are edited, grown in cultures, and then reproductively transmitted through surrogate host parents to generate live, engineered birds.

Our team would like to be able to research passenger pigeon genes for de-extinction while primordial germ cell cultures are developed, and an Australian team has developed an effective way of bypassing cell cultures for engineering birds by going directly to the primordial germ cells in the embryo.

By growing primordial germ cells in culture researchers can make genome edits sequentially without being restricted by the breeding cycle of living birds, and the cultured cells will contribute to the germ line when injected into new embryoBy growing primordial germ cells in culture researchers can make genome edits sequentially without being restricted by the breeding cycle of living birds, and the cultured cells will contribute to the germ line when injected into new embryoby the breeding cycle of living birds, and the cultured cells will contribute to the germ line when injected into new embryos.

Studies in cultured embryonic stem (ES) cells, and our analysis of zebrafish embryos have revealed that pluripotency is characterized by a unique chromatin signature.

Fused reconstructed embryos were further activated in 5 µM ionomycin for 5 min, followed by exposure to 1.9 mM 6 - dimethylaminopurine (DMAP) in synthetic oviduct fluid with amino acids (SOFaa) for 4 h. Following activation, embryos were then transferred and cultured in SOFaa.

IVC in the presence of fetal calf serum (FCS) followed by embryo transfer reduced by half the capacity of the resulting blastocysts to implant in the uterus when compared to culture without FCS.

Gardner DK, Rodriegez - Martinez H, Lane M. Fetal development after transfer is increased by replacing protein with the glycosaminoglycan hyaluronan for mouse embryo culture and transfer.

This technology requires technical skills that are typically not offered by veterinary practices and includes aspiration of immature or mature eggs from mares using ultrasound - guided transvaginal aspiration (TVA) of follicles, in vitro culture of the eggs, micromanipulation and microinjection of eggs with a single selected sperm, and embryo culture in the laboratory, with freezing, and transfer of embryos to synchronized recipient mares.