Sentences with phrase «by endothelial»

Flavonoids, including anthocyanins, seem to modulate this type of monocyte adhesion during the inflammatory process by decreasing their expression by endothelial cells.

E-selectin is expressed exclusively by endothelial cells, whereas ICAM - 1 is constitutively expressed by several cells, including endothelium and leukocytes (25).

The mediator of this effect is an odorless, colorless gas called nitric oxide, produced by the endothelial lining of every blood vessel in your body under conditions of health and pleasure, meaning sustainable aerobic exercise.

MMP - 2 and uPA are extracellular matrix remodeling ezymes expressed by endothelial cells and involved in angiogenesis.

Since invasion is dependent on extracellular matrix remodeling capabilities, this inhibitory effect strongly suggested that the two key extracellular membrane remodeling enzymes expressed by endothelial cells, namely, MMP - 2 and uPA could be other main key targets of the pharmacological action of kahweol on endothelial cells.

Identifying the factor released by endothelial cells could lead, said Stainier, to a mechanism for prompting naive, or undifferentiated, cells to develop into red blood cells, which could then be used to boost the blood supply.

Figure 2 shows sets of results for four areas of human corneal endothelial tissue; central endothelium (defined by endothelial cells within a 4 mm trephined boundary), central - intermediate endothelium (defined by endothelial cells within an 8 mm trephined boundary), intermediate - peripheral endothelium (defined by endothelial cells between the 4 mm trephine edge and 12 mm from the center of the corneal endothelium), and the peripheral endothelium (defined by the 8 mm trephined edge and 12 mm from the center of the corneal endothelium).

These results suggest that response to inflammatory cytokines by endothelial cells is not required for NS1 to cause blood vessel leakage.

They focused towards this end on the vascular growth factor Angiopoietin - 2, which is produced by endothelial cells and does not just control angiogenesis, but acts as a key responsiveness factor for endothelial cells.

These proteins most closely resembled those made by endothelial cells in the lymphatic system, not the proteins made by macrophages or other immune cells.

MIT engineers designed RNA - carrying nanoparticles (red) that can be taken up by endothelial cells (stained blue).

Not exact matches

Common wisdom in this field had held that the endothelial cells must help immune cells squeeze through by contracting themselves like small muscles, but the present study found no evidence for such contraction - based help.

Prof. Alon says: «Our study shows that the endothelial cells, which were thought to be dynamic assistants in this process of crossing of blood vessel walls, are really more responders to the «physical work» invested by the white blood cell motors and nuclei in generating gaps and crossing through blood vessels.»

Sydenham chorea was like rheumatic fever of the brain, thought to occur when rheumatic fever progresses and strep antibodies (emboldened by fever) breaches the blood - brain barrier (BBB), the tight wall of endothelial cells ordinarily there to protect the brain from the outside world.

Among dozens of mouse studies, he presented work led by Moderna Co-Founder Kenneth Chien, then at Harvard Medical School in Boston, showing that mice recovering from induced heart attacks survived longer and had stronger hearts when injected with mRNA encoding a protein that drives blood vessel formation — vascular endothelial growth factor (VEGF).

By using this technique, we demonstrated that the telomere G - tail length of leukocytes is significantly correlated with endothelial function and severity of ARWMCs,» said Professor Hidetoshi Tahara, a principal investigator of this study at the Department of Cellular and Molecular Biology, Hiroshima University.

Still, they hope to discover drugs that could put the brakes on at least some metastases by short - circuiting the ability of endothelial progenitors to respond to chemical cues sent out by tumors.

With the best - performing particles, the researchers reduced gene expression by more than 50 percent, for a dose of only 0.20 milligrams per kilogram of solution — about one - hundredth of the amount required with existing endothelial RNAi delivery vehicles.

A team from Cold Spring Harbor Laboratory in Long Island, N.Y., reports that it staved off full - blown metastasis in mice by preventing mini-tumors in the lungs from recruiting stem cells called endothelial progenitors, which assemble into blood vessels to nourish the malignancy.

By blocking these in lung endothelial cells, the researchers were able to slow lung tumor growth in mice and also reduce the spread of metastatic tumors.

The epithelium's maturation into a villus intestinal epithelium with long finger - like extensions was helped along by co-culturing human intestinal microvascular endothelial cells on the opposite side of the shared matrix - coated porous membrane in the «vascular» channel where they assembled a surrogate blood vessel with a hollow lumen through which feeding medium was flowed.

The researchers» investigations revealed that Del - 1 was expressed by at least three cell types in the bone marrow that support hematopoetic stem cells: endothelial cells, CAR cells and osteoblasts.

In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channel.

Now, a multidisciplinary research team led by David Eckmann, MD, PhD, Horatio C. Wood Professor of Anesthesiology and Critical Care at the Perelman School of Medicine at the University of Pennsylvania and professor of Bioengineering in Penn's School of Engineering and Applied Science, has found that when delivered by a microscopic transporter called a nanocarrier, steroids can access the hard - to - reach lung endothelial cells that need it most and are successful at preventing inflammation in mice.

The classical concepts of angiogenesis consider the endothelial cells within tumors as a rather passive cell population that merely reacts in response to growth factors released by the tumor cells.

To study this barrier and determine why a lack of blood flow causes it to leak, the researchers built a blood - vessel - on - a-chip model consisting of a channel lined with a layer of human endothelial cells surrounded by extracellular matrix within a microfluidic device, which allowed them to easily simulate and control the flow of blood through a vessel and evaluate the cells» responses.

«Eliminating endothelial CD146 by conditional knockout in two different mouse models of colitis significantly reduced the severity of inflammation and decreased tumor incidence and tumor progression in a mouse model of CAC,» reports lead investigator Xiyun Yan, PhD, from the Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing.

The researchers were able to increase platelet production in the bioreactor by embedding the silk with active endothelial cells and endothelial - related molecular proteins that support platelet formation.

Dengue virus NS1 triggers endothelial permeability and vascular leak that is prevented by NS1 vaccination

Lymphocytes constantly circulate through blood vessels within lymph nodes and other lymphoid organs, but only some actually enter lymphoid tissue by crossing the walls of the vessels, known as high endothelial venules (HEVs).

Earlier studies by Basu and others have shown that dopamine blocks the growth of new blood vessels in tumors by inhibiting the action of vascular endothelial growth factor - A (VEGF - A).

When a new vessel is needed, the endothelial cells direct the process by allowing the recruitment of blood cells required for neovascularization, commonly called angiogenesis.

Genentech scientists led by Mark Dennis designed an antibody with two arms — one that binds to BACE1 and inhibits its activity and one that binds to the transferrin receptor and tricks endothelial cells into transporting the antibody across the blood - brain barrier.

The anti-vascular endothelial growth factor drugs ranibizumab and aflibercept, used to treat vision loss from diabetic macular edema (DME), and approximately 20 to 30 times more expensive than bevacizumab, are not cost - effective for treatment of DME compared to bevacizumab unless their prices decrease substantially, according to a study published online by JAMA Ophthalmology.

The ability of the endothelial cells to form capillary - like structures was assessed by placing them on the solubilized basement membrane preparation Matrigel (Becton Dickinson, San Jose, CA), according to the manufacturer's instructions.

The constitutive and inducible expression of the endothelial cell adhesion molecules E-selectin, ICAM - 1, and VCAM - 1 was assessed by immunohistochemistry (Figs. 4, A — C) ⇓.

As shown in Fig. 4B, suppressive ability of miR - 7 on both transmigration through endothelial cells and Matrigel was significantly attenuated by ectopic expression of KLF4, suggesting that miR - 7 suppresses invasive ability of CSCs by blocking KLF4 expression.

The endothelial cell fraction was labeled by resuspending the pellet in 2 % DMEM containing 4 μg / ml PE - conjugated rat antimouse E-selectin mAb and 2 μg / ml FITC - conjugated rat antimouse VCAM - 1 mAb.

When primary cultures from these mice are stimulated with proinflammatory cytokines, the endothelial cell fraction responds by up - regulating the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1.

Peter Carmeliet and his team demonstrated that endothelial cells can be paralyzed by blocking glycolysis and consequently stop to form blood vessels.

Phase - contrast images of organ - derived endothelial cells (37 °C culture conditions) that had undergone two successive selection processes by flow cytometry.

The immortalized mouse brain microvascular endothelial cells (mBrEC) were supplied by Dr. Isaiah J. Fidler (M.D. Anderson Cancer Center, Houston, TX; ref.

The ability to up - regulate endothelial cell adhesion molecules after exposure to cytokines was unaffected by repeated subculturing of cells, because we noted identical staining patterns for passage 30 cells (we did attempt to extend this observation to include later passage cells).

To stain endothelial cells for adhesion molecules, a peroxidase - conjugated goat antirat IgG F (ab ′) 2 fragment was added for 45 min at room temperature and then removed by washing with PBS.

These data reveal that the tissue damage present in this SIRS model is reflected, in part, by breaks in the vasculature due to endothelial cell necroptosis and thereby predict that RIPK1 kinase inhibitors may provide clinical benefit to shock and / or sepsis patients.

Efforts to study the molecular mechanisms that mediate these complex processes in different anatomical compartments have been impeded by difficulties in the isolation and propagation of endothelial cells from different organs.

It was at this stage that highly purified populations of endothelial cells could be obtained by adjusting the gating parameters to select only the most intensely labeled cells.

Efforts to promote a selective increase in the number of endothelial cells present in primary cultures by using different media preparations (MCDB - 131, D - Valine, etc.) were largely unsuccessful.

Pilot studies using a variety of strategies suggested that this goal could be best achieved by stimulating primary organ cell cultures with TNF - α (10 ng / ml) and targeting cells expressing the endothelial cell adhesion molecules E-selectin and VCAM - 1 through a FACS - driven strategy.

By contrast, DN T cells are able to mediate DTH to donor alloantigens and induce apoptosis of donor - specific corneal endothelial cells.