Sentences with phrase «by homologous recombination»

This approach allows to obtain models in shorter timelines and with reduced costs compared to gene targeting by homologous recombination in ES cells.
Mice generated from embryonic stem cells in which ion channel genes have been mutated by homologous recombination often have a perfectly normal heart.
For more information: Cell Research Authors: Mo Li, Keiichiro Suzuki, Jing Qu, Preeti Saini, Ilir Dubova, Fei Yi, Jungmin Lee, Ignacio Sancho - Martinez, Guang - Hui Liu and Juan Carlos Izpisua Belmonte Efficient correction of hemoglobinopathy - causing mutations by homologous recombination in integration - free patient iPSCs
The precursor FP used, eqFP615, was transfected into the DT40 cell in a targeting construct that site specifically integrates by homologous recombination as previously described [12].
BRCA2 tumour suppressor protein has a recognized role in the repair of DNA double strand breaks by homologous recombination.
Gene targeting by homologous recombination in hiPS cell has proven difficult.
An independent team led by Hans Clevers at Hubrecht Institute in The Netherlands used the CRISPR / Cas9 genome editing system to correct the cystic fibrosis transmembrane conductor receptor (CFTR) by homologous recombination in cultured intestinal stem cells of patients with cystic fibrosis.
In brief, the entire ORF of the RhoH gene located in the third exon was replaced with a cassette consisting of a lacZ and neomycin resistance gene (LacZ / neo) by homologous recombination.
A, Targeting vector was designed to disrupt the RhoH gene by homologous recombination.
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