Sentences with phrase «by homologous recombination in»

For more information: Cell Research Authors: Mo Li, Keiichiro Suzuki, Jing Qu, Preeti Saini, Ilir Dubova, Fei Yi, Jungmin Lee, Ignacio Sancho - Martinez, Guang - Hui Liu and Juan Carlos Izpisua Belmonte Efficient correction of hemoglobinopathy - causing mutations by homologous recombination in integration - free patient iPSCs

Gene targeting by homologous recombination in hiPS cell has proven difficult.

This approach allows to obtain mouse models in shorter timelines and with reduced costs compared to gene targeting by homologous recombination in ES cells.

This approach allows to obtain models in shorter timelines and with reduced costs compared to gene targeting by homologous recombination in ES cells.

Not exact matches

Mice generated from embryonic stem cells in which ion channel genes have been mutated by homologous recombination often have a perfectly normal heart.

Ordinarily, a long continuous stretch of DNA would be broken up by the reshuffling of maternal and paternal chromosome segments that occurs in each new generation (known as homologous recombination).

The goal of this protocol (written in French) is to describe the steps and quality controls to prepare new stable murine embryonic stem cells lines: ① either from wild type mouse embryos to introduce a mutation by genetic targeting or homologous recombination to get a genetically modified mouse line ② or from genetically modified mouse embryos with two aims:

Promyelocytic leukemia nuclear bodies support a late step in DNA double - strand break repair by homologous recombination.

Role of Purα in the modulation of homologous recombination - directed DNA repair by HIV - 1 Tat.

Approximately 20 % of metastatic prostate cancers harbor mutations in genes required for DNA repair by homologous recombination repair (HRR) such as BRCA2.

BRCA2 tumour suppressor protein has a recognized role in the repair of DNA double strand breaks by homologous recombination.

Deltagen targeted knockouts are constructed by homologous recombination and created in - house on consistent background strains.

In brief, the entire ORF of the RhoH gene located in the third exon was replaced with a cassette consisting of a lacZ and neomycin resistance gene (LacZ / neo) by homologous recombinatioIn brief, the entire ORF of the RhoH gene located in the third exon was replaced with a cassette consisting of a lacZ and neomycin resistance gene (LacZ / neo) by homologous recombinatioin the third exon was replaced with a cassette consisting of a lacZ and neomycin resistance gene (LacZ / neo) by homologous recombination.