Sentences with phrase «by hybridization»

Methylation profiles covering the promoter regions of 20 000 genes and 400 microRNAs were generated using MeDIP followed by hybridization to microarrays.

By hybridization with fossil fuel burners (or gas turbines / reciprocating engines) time periods of low solar resource are bridged.

There are lots of other changes this represents too, such as the presence of chemicals in our food supply (pesticides, herbicides, xenoestrogens, etc) that didn't exist in the ancient diet, as well as the dramatic changes caused by hybridization of many of our foods, which creates versions of fruits and vegetables bred more for size, sweetness, and appearance as opposed to natural nutrition... this aspect has decreased micronutrient content in the modern day food supply (just look at wild blueberries vs cultivated blueberries as an example of that with wild berries coming in at more than DOUBLE the antioxidants from ORAC testing).

The results show that the new lineage was formed by the hybridization of two different species of Darwin's finches.

By hybridization we found there were regions of conservation even between bacteria and eukaryotes.

Instead, gene variants will need to be introduced by hybridization with other diverse strains of S. cerevisiae, says Borneman, such as those found in natural fermentations of African palm wine and other environmental isolates.

Meanwhile American crocodiles, which abound across Cuba, are not threatened by hybridization, Amato says.

However, microarray analysis is only capable of measuring the status of known transcripts, and expression of low - abundance mRNAs is often not detected by the hybridization - based approach.

Not exact matches

«NuMex Memorial Day» and «NuMex Thanksgiving» originated from the hybridization of «Ivory» by a dwarf plant in 1991.

«NuMex Valentine's Day,» «NuMex St. Patrick's Day,» «NuMex Halloween,» and «NuMex Christmas» are from the hybridization of» Black Prince» by «NuMex Thanksgiving» in 1995.

To accomplish his goal, García selected fourteen chile accessions growing in the Las Cruces area and by selection and hybridization began to eliminate lines with less desirable characteristics.

Indeed, I am shocked to the core to note that the hybridization of the wheat by human intervention has caused the wheat to be so deconstructed that the property of the modern wheat actually caused more harm than good to the human body.

A small study of 60 randomly selected 7 - year - old children, 31 delivered by cesarean and 29 delivered vaginally, assessed microbiota composition by determining fecal microbiota profiles using culture - independent fluorescent in situ hybridization and compared the respective effects of delivery mode on gut microbiota (12).

The SureSeq mix increases the success and accuracy of hybridization - based NGS for FFPE samples by repairing damage such as nicks and gaps, oxidized bases, blocked 3» ends, and deamination of cytosine to uracil.

The hybridization between ancient cultivated apples and M. sylvestris, followed by extensive human selection, gave us new apples that are larger and fuller in flavor, and with a crispy firmness that gives them a longer shelf life.

Unexpectedly, they show that the appearance of duplicated genes was not caused by a simple duplication of the whole genome but rather by a mating («hybridization») event between two different species.

They identified ALK - positive mesotheliomas by immunohistochemistry; confirmed with fluorescence in situ hybridization; and performed targeted next - generation sequencing of tumor DNA and RNA to get a full picture of the exact genetic rearrangement underpinning the disease.

«In the tree frog species studied by us, it is amazing that the mating and hybridizations appear to happen mainly in the Polish lowlands,» says Stöck, «while they rarely do so in Greece, where these two species meet each other as well; however, presumably since distinctly longer periods of time.»

The microbes are specifically stained by Fluorescence - In - Situ - Hybridization (FISH).

Another confounding factor in earlier studies: Researchers sampled DNA from modern purebred dogs, which are the result of generations of artificial selection and hybridization by breeders, skewing the genetic timeline of when wolves and dogs parted ways.

The test developed by Garcia for the current study used fluorescent in situ hybridization (FISH) to visualize BRAF and its fusion partner KIAA1549 inside cells, allowing researchers to see when these genes were fused together and when they were apart.

But a new study in Nature Climate Change predicts that the overall occurrence of these hybridization events between closely related species will actually be relatively low: On average, only 6.4 % of species are expected to come into geographic contact with a hybridization possibility by the end of the century.

Therefore, in 2015, the DNA was extracted from the teeth and, following hybridization capture of the mitochondrial and Y chromosome fractions, sequenced by a next generation method.

It turns out that within five to 11 generations of fish (about 25 to 50 years), the foreign genes introduced into wild populations through hybridization are removed by natural selection.

Though crossbreeding between Amazonian butterflies was not triggered by climate change, it is an example of hybridization that strengthens the survival abilities of a species.

The major hurdles flagged by WMI are the need for multiple wild populations, hybridization with coyotes, the integrity of the wolf genome, and land ownership patterns in wolf habitat.

Fraser, himself an avid fisherman, says these results provide hope for wild populations that were initially negatively affected by human - induced hybridization.

Consequently, effective conservation of native biota can be enhanced by an understanding of the ecological and evolutionary consequences of human - mediated hybridization.

After acetylation by treatment with 0.1 m triethanolamine 0.25 % acetic anhydride, pH 8.0, the tissue was prehybridized (prehybridization solution: 0.75 m NaCl, 25 mm PIPES, 25 mm EDTA, 5 × Denhardt's reagent, 0.2 % SDS, 250 μg / ml Escherichia coli tRNA, and 500 μg / ml salmon testis DNA, pH 6.8) at room temperature for 5 h. Riboprobes were incubated for 10 min at 85 °C before application onto the sections and used for hybridization at a final concentration of 500 ng / ml.

Furthermore, hybridization is exacerbated by habitat modification (Allendorf et al. 2001), so this problem will probably become more serious with increasing land use and global climate change.

This allowed the group led by Ole Seehausen (head of the Fish Ecology and Evolution department at Eawag and Professor of Aquatic Ecology at Bern University) to provide strong evidence for his theory that hybridization between divergent species, in conjunction with ecological opportunity, can facilitate rapid adaptive radiation.

In a study published in Nature Communications, they demonstrate for the first time that this rapid evolution was facilitated by earlier hybridization between two distantly related cichlid species from the Upper Nile and Congo drainage systems.

By exploring yeasts» native habitat and looking to see where else in the world they have turned up, scientists may unlock secrets of yeast genetics and hybridization with enormous economic benefit.

We addressed this issue by providing quantitative estimates of the fitness consequences of human - mediated hybridization using parentage assignment.

Now, says Peter Grant, «he might wish to redraw that tree by making connections between some of the branches, representing the hybridization and gene exchange».

Small amounts of hybridization markedly reduced fitness of male and female trout, with reproductive success sharply declining by approximately 50 per cent, with only 20 per cent admixture.

Hybridization can reduce fitness by either introducing alleles to a population that are not suited to the local environment (extrinsic outbreeding depression) or disrupting co-adapted gene complexes (intrinsic outbreeding depression)(Templeton 1986).

Guenons often form polyspecfic groups in which up to six species live side by side, however hybridization between species, while possible, is rare in undisturbed circumstances.

(C — N) Expression analysis of Acks by in situ hybridization using coronal brain sections of seizure - induced (Sz - induced)(C — H) or control Japanese honeybee workers (I — N).

(D) Cryostat section (12 µm) shows cells labeled for the retinal ganglion cell (RGC) marker, hermes, by in situ hybridization.

Resulting from the special sp orbital hybridization mediated by the Ga - d orbital in ML GaN, the strongly polarized Ga — N bond, localized charge density, and its inhomogeneous distribution induce large phonon anharmonicity and lead to the intrinsic low κ of ML GaN.

The paper, «Hybridization reveals the evolving genomic architecture of speciation,» was supported by the National Science Foundation.

The high resolution that is achieved by these techniques, particularly by microarray technologies such as array comparative genomic hybridization, is blurring the traditional distinction between cytogenetics and molecular biology.

The hybridization and processing of the GeneChips were conducted by Salk Institute's Functional Genomics Core Facility using the following systems from Affymetrix (Santa Clara, CA, USA): GeneChip ® Hybridization Oven 640, GeneChip ® Fluidics Station 450 to the wash and stain operation of Affymetrix GeneChip ® arrays, and the GeneChip ® Scahybridization and processing of the GeneChips were conducted by Salk Institute's Functional Genomics Core Facility using the following systems from Affymetrix (Santa Clara, CA, USA): GeneChip ® Hybridization Oven 640, GeneChip ® Fluidics Station 450 to the wash and stain operation of Affymetrix GeneChip ® arrays, and the GeneChip ® ScaHybridization Oven 640, GeneChip ® Fluidics Station 450 to the wash and stain operation of Affymetrix GeneChip ® arrays, and the GeneChip ® Scanner 3000 7G.

The team's work highlights this by presenting a comprehensive view of genome evolution on many different levels (e.g., differences in ploidy, aneuploidy, genetic variants, hybridization, and introgressions) that is difficult to obtain at the same scale and accuracy for other eukaryotic organisms.

Another distinctive genetic feature of mucosal melanoma is its high rate of copy number and structural variants, demonstrated both by array - based comparative genomic hybridization and whole - genome sequencing.

(B - D) CCHa2 - R mRNA was detected by in situ hybridization; signals were amplified by the TSA system.

Klein will investigate, using the genotyping by sequencing approach, the chromosomal location of potential genetic barriers affecting successful hybridization between the two.

Other efforts include array - based sequencing - by - hybridization with Callida Genomics, and simultaneous typing of SNPs on chromosomal regions undergoing LOH using customized Affymetrix tag - arrays.

Visualization of Microbiota in Tick Guts by Whole - Mount In Situ Hybridization — Caitlin E. Moss — Jove