Sentences with phrase «by immunoblot»
Levels of MEK1, MEK2, MKK3, MKK6 and actin were analyzed by immunoblot analysis.
http://journals.plos.org/
HIV - 1 p55 / p24 by immunoblot (weak reactivity).
As further evidence of BMP signaling activation, proteins isolated from zebrafish embryos were examined by immunoblotting for phosphorylated Smad1 / 5.
HuH - 7 cell extracts (lane 3) or FLAG - Sp1 - containing cell extracts (lane 4; 1.5 mg each) were immunoprecipitated with anti-FLAG antibody — conjugated beads and the immunoprecipitates were analyzed by SDS - PAGE followed by immunoblotting with anti-FLAG antibody or anti-DDX3 antibody.
After extensive washing with immunoprecipitation lysis buffer, the immunoprecipitated proteins were analyzed by immunoblotting using specific antibodies against DDX3 and FLAG epitope (ab1257, Abcam, Cambridge, United Kingdom).
Cell lysates of hippocampal tissue from the aged huAPP / PS1 and control mice were analyzed for an effect of J147 on the APP processing pathway by immunoblotting with antibodies against BACE (D) and APP (E).
After 48 hours of transfection, cell extracts were prepared and the expression level of FLAG - DDX3, p21waf1 / cip1, and α - actin was detected by immunoblotting with anti-FLAG, anti-p21waf1 / cip1, and anti-α-actin (Santa Cruz Biotechnology, Santa Cruz, CA) antibodies.
A band consistent with the entire ectodomain was observed by immunoblotting for the majority of the proteins and their expression levels were quantified.
We assayed inflammasome activation by immunoblotting and ELISA to detect IL - 1β processing and LDH release to determine pyroptosis.
The indicated cells were untreated or treated with doxycycline for 4 days followed by immunoblotting and imaging analyses.
Not exact matches
Protein immunoblot and histochemical analysis with antiserum to type I NO synthase suggest that the formation of NO in pancreatic B cells is catalyzed by an NADPH -(reduced form of nicotinamide adenine dinucleotide phosphate), Ca2 + / calmodulin - dependent type I NO synthase of about 150 kilodaltons.
Normalized protein lysates were subjected to SDS - PAGE, transferred to nitrocellulose, and immunoblotted with primary Abs from Cell Signaling Technology (Danvers, MA) at concentrations recommended by the manufacturer.
Immunoblots showed almost complete inhibition of mTOR expression (as shown in Fig. 4B) that resulted in decreased cell proliferation as assessed by MTS assay (A).
Protein was abolished as shown by Northern blot and immunoblot analysis.
Immunoblotting assays were carried out by standard procedures using Snail (Cell Signaling Technology, Inc.) and vimentin antibodies (Novus Biologicals, Littleton, CO, USA).
Immunoblots were interpreted by criteria of the Centers for Disease Control and the Association of State and Territorial Public Health Laboratory Directors [31].
Immunoblot analysis of recombinant antigens and B. burgdorferi lysates with representative sera from mice inoculated by i.p. / s.c. routes with B31 - A3 WT, ospC7 mutant, and ospC7 / ospC +4 complemented B. burgdorferi clones.
Nondenaturing 2 - dimensional gradient gel electrophoresis followed by apoA - I immunoblotting was performed on plasma from the patient and 17 control participants.20