They proliferated for a prolonged period of time (30 passages) in vitro maintaining a normal 40 XY chromosome number (supporting information Figure S1) and expressed Oct4 and Sox2, as revealed
by immunofluorescence staining (Figure 3B).
Olfactory bulb tissue sections of isochronic (control) and heterochronic old (Het - O) mice were stained
by immunofluorescence for BrdU (replicating cell marker) and neuronal neurons (NeuN) to detect newborn neurons.
The morphological features of this neurofilament, as
demonstrated by immunofluorescence and immunoperoxidase staining, and the partial characterization of the antibody are described.
We further had to test a total of eight different antibodies against phospho - AKT and six different phospho - MAPK antibodies, before being able to detect BDNF
signalling by immunofluorescence.
In addition, the contribution of iPS cells to the livers of chimeric mice was
evaluated by immunofluorescence staining using antibodies against CD31 (a marker of vascular endothelial cells) and AFP (a marker of liver cells).
Additional blinded screening of the 51 cases and 53 controls at the Robert Koch Institute using an ELISA employing recombinant Gag and Env XMRV proteins identified weak seroreactivity in one CFS case and a healthy control, which was not
confirmed by immunofluorescence.
In later stages, the NLS - PCP - GFP fluorescent signal is reduced at the posterior pole and Oskar protein (blue) is
detected by immunofluorescence, consistent with translation of a portion of the transcripts (see Halstead *, Lionnet *, Wilbertz *, Wippich * et al., Science 2015).
After binding, cells were washed once (carefully using a 200 μl pipette to remove the medium) with RPMI with 0.5 % BSA solution and adherent cells were
analyzed by immunofluorescence microscopy or bright field microscopy for quantification.
By immunofluorescence microscopy, Thy1 - PE - labeled CD3ε + cells were observed in the SCS and in nearby lymphatic sinuses, and few labeled cells were detected within the LN parenchyma, confirming that footpad injection of PE - conjugated antibody led to preferential labeling of lymph - exposed LN cells (Figure 3E).