Sentences with phrase «by kahweol»

The global morphological features (including centrifugal growth of the peripheral vessels - relative to the position of the disc -, avoiding the treated area, with an overall decrease in the vascular density) elicited by kahweol treatment are also in agreement with those previously observed for other anti-angiogenic compounds.

In the CAM assay, the inhibitory doses exhibited by kahweol are similar to those of other anti-angiogenic compounds found by us to inhibit angiogenesis in the CAM assay [10], [19], and much lower than those of other anti-angiogenic compounds [21].

Not exact matches

Figure 6 shows the effects of 75 µM kahweol on endothelial cell migration, as determined by the «wound healing» assay, after 8 and 24 h of treatment.

To get new, additional insights on the features of kahweol as an anti-angiogenic compound, we carried out a complete set of in vitro assays previously used by us to characterize the anti-angiogenic effects of other compounds from natural sources, including aeroplysinin - 1, homocysteine, ursolic acid, puupehenone, hypericin, hyperforin and aloe - emodin, among others [11], [12], [13], [19], [20], [21].

Our results reinforce the potential pharmacological interest of kahweol, as suggested by its anti-angiogenic and anti-inflammatory effects.

Invading controls and 25 and 75 µM kahweol - treated HUVEC cell count values are represented by using diamonds, squares and triangles, respectively.

On the other hand, the present research work shows a confirmatory evidence of the potential of kahweol to inhibit in vivo angiogenesis, by using another completely independent model system, namely, that of genetically modified zebrafish.

Since invasion is dependent on extracellular matrix remodeling capabilities, this inhibitory effect strongly suggested that the two key extracellular membrane remodeling enzymes expressed by endothelial cells, namely, MMP - 2 and uPA could be other main key targets of the pharmacological action of kahweol on endothelial cells.

The CAM and zebrafish in vivo assays and the ex vivo mouse aortic ring assay clearly identify kahweol as a new anti-angiogenic compound, but gives no information on which specific steps of angiogenesis are targeted by this compound.

We also demonstrate the inhibitory effect of kahweol on the endothelial cell potential to remodel extracellular matrix by targeting two key molecules involved in the process, MMP - 2 and uPA.

Figure 9 (A and B) shows that kahweol inhibits in a dose dependent manner the expression of COX - 2 protein by HUVEC.

C) Quantification of the amount of MCP - 1 secreted by HUVEC after a 24 h treatment in the presence of different concentrations of kahweol.

Data obtained on the effects of kahweol on endothelial cell invasion (as determined by a continuous fluorescent assay) clearly show that kahweol induces an anti-invasive effect in HUVEC in a dose - dependent manner (Figure 7).

A third line of evidence showing the potential of kahweol to inhibit overall angiogenesis is provided by the ex vivo model of the mouse aortic ring assay.

B) In situ determination of kahweol effects on HT - 1080 gelatinases, as determined by gelatin zymography with the presence of kahweol in the incubation substrate buffer.

It is believed that kahweol and cafestol palmitate increase the liver's production of glutathione, the master antioxidant, by as much as 700 %.