We offer quality custom generation of a wide range of mouse models developed
by microinjection of Sv129 / Pas or C57BL / 6N ES cells into blastocysts or by DNA pronuclear injection.
RNAi made
by microinjections resulted in ∼ 52 % reduced progeny laid in the first 24 h after microinjections and after 48 h ∼ 28 % reduction in progeny laid compared to controls (n = 260, n = 550, for day one and n = 1,000, n = 1,400 for day two).
W01A8.1 RNAi made
by microinjections and feeding resulted in a significantly smaller brood size, with approximately 30 % less progeny.
Not exact matches
This «
microinjection» approach was pioneered
by a team in Hawaii to produce Cumulina, the first cloned mouse (ScienceNOW, 22 July 1998).
When overexpressed together in developing Xenopus embryos
by RNA
microinjection, these genes can induce eye - like structures as defined
by the expression of markers for some retinal cell classes [7].
Generation of genetically modified mice
by random insertion of a transgene can be performed
by pronuclear DNA
microinjection.
17 Capecchi MR. High Efficiency Transformation
By Direct
Microinjection of DNA into Cultured Mammalian Cells.
High efficiency transformation
by direct
microinjection of DNA into cultured mammalian cells.
This technology requires technical skills that are typically not offered
by veterinary practices and includes aspiration of immature or mature eggs from mares using ultrasound - guided transvaginal aspiration (TVA) of follicles, in vitro culture of the eggs, micromanipulation and
microinjection of eggs with a single selected sperm, and embryo culture in the laboratory, with freezing, and transfer of embryos to synchronized recipient mares.