Sentences with phrase «by nucleases»

Just as important, the UI probe has been chemically modified so that it's shredded only by the staph bacteria's nuclease and not by a nuclease secreted by normal, healthy cells.

The use of viral vector for mouse mutagenesis and / or for delivery of gene editing by nuclease (F. Piguet, IGBMC)

Our studies establish that the H19 and U2af1 - rs1 DMRs have a specialized organization of chromatin characterized by nuclease hypersensitivity at the unmethylated chromosome.

Not exact matches

These risks and uncertainties include: Gilead's ability to achieve its anticipated full year 2018 financial results; Gilead's ability to sustain growth in revenues for its antiviral and other programs; the risk that private and public payers may be reluctant to provide, or continue to provide, coverage or reimbursement for new products, including Vosevi, Yescarta, Epclusa, Harvoni, Genvoya, Odefsey, Descovy, Biktarvy and Vemlidy ®; austerity measures in European countries that may increase the amount of discount required on Gilead's products; an increase in discounts, chargebacks and rebates due to ongoing contracts and future negotiations with commercial and government payers; a larger than anticipated shift in payer mix to more highly discounted payer segments and geographic regions and decreases in treatment duration; availability of funding for state AIDS Drug Assistance Programs (ADAPs); continued fluctuations in ADAP purchases driven by federal and state grant cycles which may not mirror patient demand and may cause fluctuations in Gilead's earnings; market share and price erosion caused by the introduction of generic versions of Viread and Truvada, an uncertain global macroeconomic environment; and potential amendments to the Affordable Care Act or other government action that could have the effect of lowering prices or reducing the number of insured patients; the possibility of unfavorable results from clinical trials involving investigational compounds; Gilead's ability to initiate clinical trials in its currently anticipated timeframes; the levels of inventory held by wholesalers and retailers which may cause fluctuations in Gilead's earnings; Kite's ability to develop and commercialize cell therapies utilizing the zinc finger nuclease technology platform and realize the benefits of the Sangamo partnership; Gilead's ability to submit new drug applications for new product candidates in the timelines currently anticipated; Gilead's ability to receive regulatory approvals in a timely manner or at all, for new and current products, including Biktarvy; Gilead's ability to successfully commercialize its products, including Biktarvy; the risk that physicians and patients may not see advantages of these products over other therapies and may therefore be reluctant to prescribe the products; Gilead's ability to successfully develop its hematology / oncology and inflammation / respiratory programs; safety and efficacy data from clinical studies may not warrant further development of Gilead's product candidates, including GS - 9620 and Yescarta in combination with Pfizer's utomilumab; Gilead's ability to pay dividends or complete its share repurchase program due to changes in its stock price, corporate or other market conditions; fluctuations in the foreign exchange rate of the U.S. dollar that may cause an unfavorable foreign currency exchange impact on Gilead's future revenues and pre-tax earnings; and other risks identified from time to time in Gilead's reports filed with the U.S. Securities and Exchange Commission (the SEC).

By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus.

GUIDE - seq enables genome - wide profiling of off - target cleavage by CRISPR - Cas nucleases.

They digested human genomic DNA using Cas9 nucleases in a test tube, which was then subjected by whole genome sequencing.

Furthermore, by adding guanine nucleotides at the end of sgRNA (single guided RNA) that composes CRISPR - Cas9, they have successfully created this highly - developed programmable nuclease, which has no measurable off - target effects in the human genome.

Staplhylococcal nuclease undergoes a reversible structural transition between ph3 and 4 which be mesured by changes in tryptoham fluorescence.

Just this week, for example, a team led by Feng Zhang of the Broad Institute of Harvard and MIT, one of the pioneers of the method, published a paper in Science on engineering the nuclease part of CRISPR so that it more accurately cuts the intended DNA target.

Moreover, they can be degraded quickly by special enzymes (nucleases) that are present in bodily fluids such as saliva or blood that digest foreign DNA.

A similar mutation, detected by S1 nuclease mapping of LDL receptor messenger RNA, occurred in a patient with familial hypercholesterolemia whose receptor also fails to be transported to the cell surface.

«If the probe gets cleaved by serum nucleases, then our probe would be lit up all over the bloodstream.

What's more, say the researchers, the cancer - causing effects of off - target deletions mistakenly created by the V (D) J enzyme need to be considered in designing site - specific enzymes for genome modification such as zinc - finger nucleases, TALENS, or CRISPRs.

In tests, nucleases (or enzymes) produced by the staph bacteria cleave the particles, like a warrior wielding a sword.

McNamara acknowledges previous research by Arthur Arnone, UI professor emeritus in biochemistry, who was the first to define the structure of the S. aureus nuclease.

But since it's split only by staph nucleases, then we can pinpoint where the staph bacteria are active.»

Moreover, it was confirmed that, compared to previous nuclease models, cytotoxicity is significantly reduced by modifying the DNA without cutting.

The point mutation was induced by forming a synthetic complex through removal of nuclease activity from the CRISPR system — a technique using artificial nuclease — and addition of deaminase, a deaminizing (base - modifying) enzyme, and then expressing it in yeasts and mammalian cells.

Deaminase is attached by a linker to nuclease — deficient CRISPR / Cas9.

In an effort to combine the respective advantages of the AAV and CRISPR - based approaches, several groups have recently worked to enhance the efficiency of AAV - based gene editing via the introduction of a double strand break by using a targeted nuclease.

Together the stable disruption of CXCR4 as determined by both the surveyor nuclease assay and flow cytometry suggests that CXCR4 disruption did not negatively impact cell viability or growth in humanized NSG mice over a two - month period.

Cell growth was monitored every 48 hours post-stimulation for approximately two weeks and the efficiency of CXCR4 disruption was assessed at day five post-transduction by both the Surveyor nuclease assay and by deep - sequencing of the CXCR4 target site.

In the presence of Bk132, treatment with X4 - ZFNs conferred protection at 14 d.p.i (p =.05); however, this protection wanes by 34 d.p.i. (p =.88)(B) Cxcr4 disruption frequency was assessed by the surveyor nuclease assay in both peripheral blood (p <.001) and spleen (p <.001).

Here we describe engineering a pair of zinc finger nucleases that, when introduced into human T cells, efficiently disrupt cxcr4 by cleavage and error - prone non-homologous DNA end - joining.

Previously we engineered zinc - finger nucleases (ZFNs) to specifically disrupt the CCR5 gene in primary human T cells, the predominant cell type infected and killed by HIV.

Upon binding of both X4 - ZFNs, the FokI nuclease cleavage domains dimerize and then generate a double strand break that can subsequently be repaired by error - prone NHEJ resulting in mutations targeted to the cleavage site that can include missense mutations, deletions and insertions (Figure 1).

H. Efficient CRISPR / Cas9 - Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease.

Provasi E, Genovese P, Lombardo A, Magnani Z, Liu PQ, Reik A, Chu V, Paschon DE, Zhang L, Kuball J, Camisa B, Bondanza A, Casorati G, Ponzoni M, Ciceri F, Bordignon C, Greenberg PD, Holmes MC, Gregory PD, Naldini L, Bonini C. Editing T cell specificity towards leukemia by zinc finger nucleases and lentiviral gene transfer.

A few virus particles have learned to circumvent the CRISPR / Cas defense by generating a single point mutation in the PAM sequence, preventing the Cas nucleases from re-identifying it (3).

Using X-ray crystallography — a method in which patterns formed by high - powered X-rays shot through a crystal of the molecule reveal its atomic structure — the scientists found that the shape of the binding site in the mZuc protein was consistent with its identity as a nuclease.

Genetic Engineering and Model Validation: engineering and generation of customised genetically modified mouse models, generation of genetically modified ES cells, genotyping, analysis of gene expression, model validation by molecular analysis, CRISPR / Cas9 nucleases technology

In MMEJ pathway, we achieve efficient gene disruption in human cell lines and animals by developing a computer program that assists the choice of nuclease target sites based on microhomology prediction.

Programmable nucleases, ZFN, TALEN and RGENs enable gene knockout in cultured cells and organisms by producing site - specific DNA double - strand breaks, whose repair via error - prone non-homologous end joining (NHEJ) or microhomology - mediated end joining (MMEJ) gives rise to frameshift mutations.

Using Surveyor nuclease followed by gel electrophoresis, these mismatches are indicated by the presence of DNA cleavage products.

The nucleases were capable of pushing out an undesirable trait in a plant — such as a gene for a chemical that acted as an environmental pollutant when consumed by livestock — and replacing it with a desirable trait, such as herbicide resistance, in a single shot.

The enzyme activities of Caf1 and Ccr4 are both required for deadenylation by the human Ccr4 — Not nuclease module

Correction of dystrophin expression in cells from Duchenne muscular dystrophy patients through genomic excision of exon 51 by zinc finger nucleases.