Sentences with phrase «by retroviral»

These cells were immortalized by retroviral transduction of the SV40 T antigen and Telomerase reverse transcriptase (TERT), the catalytic subunit of the telomerase complex [4], and then termed HMLE (Human Mammary with Large T and TERT).

The symposium features presentations by Philippa Marrack and John Kappler talking on the T cell repertoire; William Paul on interleukin 4 as a prototypic immunoregulatory cytokine; Timothy Springer on lymphocyte trafficking; Pamela Bjorkman on structural studies of MHC and MHC - related proteins, and Jack Strominger on peptide presentation by class I and II MHC proteins; Thierry Boon on genes coding for tumor rejection antigens, including the first tumor antigen, MAGE - 1; and Philip Greenberg on the modification of T cells for adoptive therapy by retroviral - mediated gene insertion Since then, the symposia series has attracted leading immunologists in the cancer vaccine and antibody fields, providing them with a comprehensive view of the promises and challenges in the development of cancer immunotherapies.

The study, «VlincRNAs controlled by retroviral elements are a hallmark of pluripotency and cancer» found that novel non-coding parts of the human genome known as vlincRNAs (very long intergenic, non-coding RNAs) triggered by ancient viruses, participate in the biology of stem cells, and in the development of cancer.

Not exact matches

Conditional knockout of Uhrf1, in a region of the forebrain, led to the activation of endogenous retroviral elements (ERV) in the genome, which are otherwise silenced by methylation.

In a paper published in August in Science, scientists addressed this potential biohazard by knocking out the retroviral DNA with the gene editing technology CRISPR - Cas9.

While retroviruses generally only infect dividing cells (because their access to the host genome is thought to rely on the breakdown of the nuclear envelope that occurs in mitosis [Roe et al., 1993]-RRB-, lentiviruses are a genus of the retroviral family that can infect non-dividing cells (possibly through the use of nuclear localization signals by the viral components [Bukrinsky et al., 1992]-RRB-, and thus, offer an advantage when transducing certain cell types that exhibit limited cell division (e.g., neurons).

Genetic Alterations: Disruption caused by insertion of the retroviral gene trap vector ROSAFARY.

HEK293T cells were transfected with the following retroviral or lentiviral constructs, with their packaging vectors indicated in the parentheses, using FuGENE 6 reagent (Promega): pMX - puro - STAT5A * (provided by T. Kitamura, University of Tokyo; pCMV - Gag - Pol and pCMV - VSV - G); pLenti - EF - FH - TAZ - S89A (psPAX2 and pVSV - G); PLKO - NS and shTAZ lentiviral construct (provided by C. Linardic, Duke University; pCMV - Rev, pCVM - VSVG; pMDL).

The novel reprogramming approach employed the retroviral transduction of Oct4, Sox2, and Klf4 in mouse embryonic and adult fibroblasts followed by treatment with the GSK3β Inhibitor BIO and the pleiotropic cytokine Oncostatin M (OSM).

This report suggests it may be possible to reprogram human NSCs into iPS cells by OCT3 / 4 and kenpaullone, further minimizing the undesired effects of utilizing retroviral vectors.

All JAK1 and JAK2 mutants were generated using QuickChange XL II site - directed mutagenesis kit (Stratagene) and subcloned into the pMX - GFP (for JAK1) or pMEGIX - GFP (for JAK2) biscistronic retroviral vector upstream of the IRES as previously described.17 — 19 The mutated JAK1 and JAK2 constructs were verified by sequencing the full - length JAK1 and JAK2 using DYEnamic ET Dye Terminator Kit (Amersham Biosciences).

In the original identification of XMRV in prostate cancer stromal cells, Urisman et al. [3] confirmed by sequence analysis that XMRV is not a laboratory contaminant, as is often the case with claims of new retroviral associations with disease.

Having been excited by the potential of retroviral gene transfer as a therapeutic, it has been a fascinating opportunity to get to grips with the various processes in the lab; particularly more recently when I have spent time working independently to optimise the process in various cell subsets.

Feline leukemia is a retroviral disease caused by the feline leukemia virus (FeLV).

Feline Senior Wellness Checklist Comprehensive Physical Exam (recommended twice yearly) Vaccinations (Fvrcp, Felv, Rabies) * Intestinal parasite test (fecal) Retroviral testing if indicated Senior Wellness Bloodscreen and Urinalysis recommended yearly (Chem 27, CBC, thyroid, UA) + / - viral testing Blood Pressure Screening - available at associate hospital * vaccine schedule protocol tailored to each individual patient based on accepted guidelines by AAHA / AAFP