Sentences with phrase «by subcloning»

STAT2 full - length cDNA expression plasmid was obtained by subcloning the insert of pBlueScript - STAT2 (from Dr. J.E. Darnell, Jr.) to the vector pcDNA3.1.

Psin - miR7 - 2 plasmid was constructed by subcloning PCR product from pCDH - CMV - MCS - EF1 - copGFP - miR7 - 2.

Not exact matches

All JAK1 and JAK2 mutants were generated using QuickChange XL II site - directed mutagenesis kit (Stratagene) and subcloned into the pMX - GFP (for JAK1) or pMEGIX - GFP (for JAK2) biscistronic retroviral vector upstream of the IRES as previously described.17 — 19 The mutated JAK1 and JAK2 constructs were verified by sequencing the full - length JAK1 and JAK2 using DYEnamic ET Dye Terminator Kit (Amersham Biosciences).

Protein coding sequences were flanked with unique NotI and AscI restriction sites and subcloned into a derivative of the pTT3 expression plasmid between a 5» mouse variable κ light chain signal peptide [32], and a 3» tag consisting of the rat Cd4 domains 3 and 4 followed by an enzymatic biotinylation sequence and a hexahistidine tag [33].