Sentences with phrase «by the electron microscopy»

Single particles of the mediator of transcriptional regulation (Mediator) and of RNA polymerase II holoenzyme were revealed by electron microscopy and image processing.

Analysis of the membrane - bound form of annexin V by electron microscopy revealed no differences between the wild type and mutant.

A new support for biological specimens improves the resolution that can be achieved by electron microscopy.

Members of Paul Sullam's lab at UCSF provided the clinical perspective, members of Maofu Liao's lab at Harvard characterized the targeting complex by electron microscopy, and members of Parastoo Azadi's lab at Georgia analyzed the sugar modifications.

Fourth, the finding of intranuclear inclusions in diseased tissues, as well as direct visualization of adenoviral - like particles (TMAdV) in lung alveoli by electron microscopy (Figs. 1D - 2 to 1D - 4), support a primary role for TMAdV in the pathogenesis of tissue injury in affected monkeys.

Advanced fluorescence microscopy will be complemented by electron microscopy approaches for ultrastructural characterization of cellular microcompartments.

«Eggshells are notoriously difficult to study by traditional means, because they easily break when we try to make a thin slice for imaging by electron microscopy,» says McKee, who is also a professor in McGill's Department of Anatomy and Cell Biology.

Right eyes were investigated by electron microscopy, left eyes were used for immunocytochemistry and fluorescence light microscopy.

In an ultrathin section of infected DEF cells obtained 24 hrs post-infection, dense particles measuring 30 to 40 nm in diameter were found within cytoplasmic vesicles in large numbers (Fig. 4E) by electron microscopy (EM).

Müller cell apical processes appear to be lost in regions lacking adherens junctions in these mice, as demonstrated by absent or reduced CD44 labeling and by electron microscopy.

To analyze the ultrastructural changes of adipocytes accompanying this functional transformation, we examined the fat tissue by electron microscopy.

Intestinal permeability was assessed by Ussing chamber; epithelial cell (EC) ultra-structure by electron microscopy; RNA expression of genes coding for junctional proteins by Q - real - time PCR; immune response by in - vitro antigen - specific T - cell proliferation and cytokine analysis by cytometric bead array; intestinal microbiota by fluorescence in situ hybridization and analysis of systemic antibodies against intestinal microbiota by surface staining of live bacteria with serum followed by FACS analysis.

This is followed by the formation of giant cytoplasmic vacuoles in acinar cells, visible only by electron microscopy.

Not exact matches

A detailed view of TRPM8's structure, obtained using cryo - electron microscopy, was published by a different research group online December 7 in Science.

In this Review, we highlight recent developments and inventions made by introducing the fourth dimension of time in electron microscopy.

Molecules such as proteins are surrounded by water that helps them maintain their structures, but electron microscopy dried up the water.

Structure of the monomeric yeast ATP synthase, shown as a surface representation, as determined by cryo - electron microscopy.

Now a research team led by Dr. Peter Baum and Dr. Yuya Morimoto at LMU Munich and the Max Planck Institute for Quantum Optics (MPQ) has developed a new mode of electron microscopy, which enables one to observe this fundamental interaction in real time and real space.

Through a combination of high - resolution cryo - electron microscopy (cryo - EM) and a unique methodology for image analysis, a team of researchers with Berkeley Lab and the University of California (UC) Berkeley has produced an atomic view of microtubules that enabled them to identify the crucial role played by a family of end - binding (EB) proteins in regulating microtubule dynamic instability.

By means of environmental transmission electron microscopy (ETEM), structural modifications on the atomic level of the material were visualized.

Hochstein and her collaborators from MSU, the University of California, Los Angeles, and the Max Planck Institute of Biochemistry in Germany learned more about the structure of the Acidianus virus by using a combination of cryo - electron microscopy and X-ray crystallography.

This achievement has been made possible by using high - resolution cryo - electron microscopy, a technique brought to the CNIO thanks to Óscar Llorca, director of the Structural Biology Programme and lead author on the paper published in Nature Communications.

A pyrenoid (blue) is seen in a cross-section of an algal cell by false - colored electron microscopy.

In this false - color scanning electron microscopy image, thermal conductivity was measured by transporting heat from the suspended heat source pad (red) to the sensing pad (blue).

The structure of RuO2 (110) and the mechanism for catalytic carbon monoxide oxidation on this surface were studied by low - energy electron diffraction, scanning tunneling microscopy, and density - functional calculations.

These are Staphylococcus aureus cells («golden staph») observed by scanning electron microscopy

Sirohi et al. present a near - atomic - resolution structure of mature Zika virus determined by cryo - electron microscopy.

By using thousands of images, they reconstructed a high - resolution cryo - electron microscopy structure of the Zika virus.

We present the 3.8 angstrom resolution structure of mature Zika virus, determined by cryo — electron microscopy (cryo - EM).

An international team led by researchers from the U.S. Department of Energy's Lawrence Berkeley National Laboratory (Berkeley Lab) used advanced techniques in electron microscopy to show how the ratio of materials that make up a lithium - ion battery electrode affects its structure at the atomic level, and how the surface is very different from the rest of the material.

Electron microscopy images of the porous graphene - based structure created by diffusion driven layer - by - layer assembly.

Much like in an old tube television where a beam of electrons moves over a phosphor screen to create images, the new microscopy technique works by scanning a beam of electrons over a sample that has been coated with specially engineered quantum dots.

Atomic structure of sensitive battery materials and interfaces revealed by cryo — electron microscopy

In microscopy much effort is invested in reducing the impact of light or electron beam — the so - called observer effect» — on the sample to ensure that the images represent truly pristine structures, unaffected by the process of measurement.

The research was carried out by experts in synthetic and theoretical chemistry, materials and electron microscopy and builds on Professor Khlobystov's concept of carbon nano test tubes (World's tiniest test tubes, Guinness Book of World Records 2005), where the nanotube acts as a container for molecules.

I had completed a postdoc with Prof. Michael Rossmann during which I studied virus - receptor relationships by using x-ray crystallography and electron microscopy.

The ORNL - Uppsala team developed the technique by rethinking a cornerstone of electron microscopy known as aberration correction.

To examine the mechanisms of this down - regulation, thin - section electron microscopy was combined with immunolabeling by gold - conjugated monoclonal antibodies specific to apCAM.

Scanning probe microscopy (SPM) resolves only surface features, and transmission electron microscopy is limited to samples readily traversed by electrons.

This was also corroborated by their ultrastructures obtained with electron microscopy.

The TSRI laboratories of Professor Erica Ollmann Saphire and Assistant Professor Andrew Ward are studying the structures of these antibodies using techniques called electron microscopy, which creates high - resolution images by hitting samples with electrons, and X-ray crystallography, which determines the atomic structure of crystalline arrays of proteins.

By using a powerful imaging technique known as cryo - electron microscopy, however, Campbell and her colleagues were able to figure out exactly how the antibiotic throws a wrench into RNAP.

Recombinant proteins containing tetracysteine tags can be successively labeled in living cells with different colors of biarsenical fluorophores so that older and younger protein molecules can be sharply distinguished by both fluorescence and electron microscopy.

By combining scanning electron microscopy with x-ray computer tomography to examine the bead's microstructure and chemistry, a British research team determined that the iron possessed a structural and microchemical signature known as the Widmanstätten pattern that is unique to weathered iron meteorites.

Scanning electron microscopy images for showing sugar blowing process: glucose were polymerized and blown by released ammonia into melanoidin bubbles in heating, which bubbles were finally converted into strutted graphene containing mono - / few - layered graphene membranes and graphitic struts.

However, these findings are incompatible with the results of efforts to locate individual ribosomal proteins by immune electron microscopy and triangulation with interprotein distance measurements.

Using electron microscopy to look at thousands of ultrathin brain slices taken from awake and sleeping mice, they found that after sleep, the size of most synapses — specifically, the surface area where two neurons touch each other — shrank by about 18 percent.

The detection of the clusters by an independent group using cryo - electron microscopy helped convince the community, Cölfen adds.

Selective imaging by correlated optical and electron microscopy of protein molecules of known ages will clarify fundamental processes of protein trafficking in situ.

The authors also employed other advanced imaging techniques — including electron microscopy and super-resolution imaging — to discover that the formation, and subsequent loosening, of these contacts is regulated by a lysosomal protein called RAB7.