Sentences with phrase «c splenocytes»
Balb / c splenocytes were used as third - party responder cells assuming that these would induce the same degree of T cell proliferation in tolerant and control animals.
http://journals.plos.org/
All three groups of animals responded equally to third - party Balb / c splenocytes.
Not exact matches
Six weeks postinfection, splenocytes were analyzed for gp33 - LCMV — specific total CD8 + T cells (B) or short - lived effector cells and memory precursor effector cells (C).
(C) Splenocytes were stained for CD8, CD4, CD44, and CD62L, and the percentage and absolute number of CD8 + CD44hi cells were determined.
(C) Sorted Lin - CD127 + cells from Rag1 - / - splenocytes were pretreated with DMSO or indicated concentrations of PD98059 or SB203580 as in A and analyzed for IL - 22 secretion by ELISA.
(A-C) Rag1 - / - splenocytes were pretreated with (A) either lethal toxin (LeTx, lethal factor + protective antigen) or lethal factor (LF)(1 μg / ml) or (B) increasing doses of lethal toxin or (C) with enzymatic mutant toxin (E687C) for 3 hr followed by IL - 23 (50 ng / ml) stimulation for 18 hr.
(C) In vitro expanded human Lin - CD127 + cells or (D) mouse Lin - CD127 + c - Kit + Thy1.2 + cells expanded from splenocytes of Rag1 - / - mice were treated with lethal toxin for 2 hr followed by IL - 23 stimulation for 30 minsC) In vitro expanded human Lin - CD127 + cells or (D) mouse Lin - CD127 + c - Kit + Thy1.2 + cells expanded from splenocytes of Rag1 - / - mice were treated with lethal toxin for 2 hr followed by IL - 23 stimulation for 30 minsc - Kit + Thy1.2 + cells expanded from splenocytes of Rag1 - / - mice were treated with lethal toxin for 2 hr followed by IL - 23 stimulation for 30 mins.
For mouse ILC3s, splenocytes from Rag1 - / - mice were sorted (Lin -(CD3, CD45R, CD11c, GR - 1, NK1.1) c - kit + Thy1.2 +) and cultured as described for human cells using recombinant mouse cytokines.
The CD8 + or CD4 + T cell lines were established in vitro by stimulating splenocytes from Gasp + / + and Gasp − / − mice after depleting CD4 + or CD8 + cells, with BALB / c (allogeneic) splenocytes or syngeneic splenocytes in the presence of 2C11 (1 μg / mL).