Sentences with phrase «calcium imaging»
"Calcium imaging" is a technique used to study and visualize the activity of cells. It involves using special fluorescent dyes that can detect changes in calcium levels, which is important for signaling and communication within cells. By measuring these changes, scientists can better understand how cells work and communicate with each other. Full definition
Because calcium imaging is widely used to identify cells in tissues throughout the body, researchers studying other systems will be able to benefit from the published methodology.
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Rigosi, E., Haase, A., Rath, L., Anfora, G., Vallortigara, G. & Szyszka, P. (2015), Asymmetric neural coding revealed by in vivo calcium imaging in the honey bee brain.
Explaination on one of Elisa Rigosi's thesis projects published in Rigosi et al. 2015, Asymmetric neural coding revealed by in vivo calcium imaging in the honeybee brain.
Two - photon calcium imaging of retino - recipient midbrain regions isolated the optic tectum as an important center processing looming stimuli, with ensemble activity encoding the critical image size determining escape latency.
We combined two - photon calcium imaging in head - fixed flying flies with optogenetics to overwrite the existing population representation with an artificial one, which was then maintained by the circuit with naturalistic dynamics.
To image the neurons firing, the researchers used a technique called calcium imaging that creates fluorescence in response to the influx of calcium that occurs when a neuron fires.
«Using calcium imaging on zebrafish, we were able to visualize an on - going process of memory consolidation for the first time.
Amongst others, we have put forward a two - photon microscopy technique based on light - sculpting that has enabled the first whole - brain calcium imaging in C. elegans.
Calcium imaging revealed sustained intracellular calcium rise upon sensory stimulation.
While powerful and widely used, calcium imaging alone is too slow to generate the kind of real - time map that the BAM project requires, says Michael Roukes of the California Institute of Technology in Pasadena.
Calcium imaging takes advantage of the fact that calcium ions enter neurons upon neural activation.
Building on traditional calcium imaging and recent optogenetic techniques for manipulating neuronal activity, the Cal - Light system links fluorescent gene expression with both activity and light.
Calcium imaging also showed that the neurons in the organoid were not firing sparsely, with isolated activity, as in cultured brain organoids, but in synchronized patterns, suggesting an active neuronal network was developing.
Caroline joined the Biophotonics group as a post doc in 2013 to implement confocal light - sheet microscopy for structural whole mouse brain imaging and then later functional calcium imaging in Zebrafish.
In vivo mapping of brain activity is implemented via calcium imaging techniques at high temporal resolution.
Recently, we extended our imaging methods to the scattering tissue domain and demonstrated fast volumetric calcium imaging across the majority of a cortical column in the mouse.
In vivo calcium imaging of the flies» brains stimulated with citrus enabled the researchers to identify the corresponding odorant receptor.
But using a technique called calcium imaging, Aoki et al. were able to visualize for the first time the activity of the whole zebrafish brain during memory retrieval.
Explaination of one of Elisa Rigosi's thesis projects published in Rigosi et al. 2015, Asymmetric neural coding revealed by in vivo calcium imaging in the honeybee brain.
Following up, Styr repeated the experiment in the lab using calcium imaging, which can monitor the activity of visually identifiable neurons.
With a technique known as two - photon calcium imaging, the scientists were then able to visualize the activity of mPOA neurons in awake, behaving female mice.
His team was able to make this discovery because of something rather rare in immunology labs: the additional ability to do both electrophysiology — the study of the flow of electrical currents in cells and tissues — and calcium imaging in living cells.
The type III salty / sour taste cells were identified using a method called calcium imaging, marking the first time that RNA - seq has been successfully used on single cells characterized using this technique.
Calcium imaging is a common technique for measuring the activity of single neurons.
The researchers used two - photon calcium imaging and patch - clamp electrophysiology, two sophisticated technologies that allowed the researchers to record the signals from individual brain cells.
Up until now, researchers who wanted to observe neuronal activity in real - time often used a technique called calcium imaging.
We will use molecular, histological, calcium imaging and electrophysiological methods to characterize the sensitivity of these channels to membrane stretch by mimicking the effect of IOP on RGC perikarya and axons.
In my current research, I investigate the sublethal effects of neonicotinoids on olfactory processing and learning using in - vivo calcium imaging.
This new method, called second harmonic generation (SHG), developed in collaboration with Columbia Chemistry Professor Ken Eisenthal, is an advance on the older technique of calcium imaging (CI), also pioneered by Yuste, which records the electrical activity (firing) of neurons indirectly by detecting the calcium that enters them as they send or receive signals.
Nobel laureate Richard Axel is using two - photon microscopy and calcium imaging to determine how specific odorants are represented at various relay points in the olfactory system.
Now I am expanding my investigation on the olfactory system of the honeybee, performing in vivo calcium imaging and behavioral assays to study the neurobiological basis of the associative learning and memory.
The results from the calcium imaging experiments using brain explants and IPC - specific CCHa2 - R knockdown strongly suggest that CCHa2 crosses the blood - brain barrier (BBB) to regulate the IPCs, although the underlying mechanism remains elusive.
Because Dr. Desai's lab can do both electrophysiology and calcium imaging in immune cells, he gets requests from immunologists all over — so many he can't keep up.
To clarify whether CCHa2 directly signals to the brain, calcium imaging was performed using ex vivo culture of larval brains expressing the fluorescent calcium sensor GCaMP6s [31] in the IPCs.
The lab studies the pathways from sensory input to behavioral output by quantitative analysis of behavior under well - defined conditions, genetic manipulation of animals or individual neuronal cells, and calcium imaging from neurons in living animals.
Methods: We used primary cultures of post-weaning murine mediobasal hypothalamic neurons, hypothalamic neurons derived from human induced pluripotent stem cells, and calcium imaging to characterize rapid neuronal responses to physiological changes in extracellular l - Leucine concentration.
In his lab we use multidisciplinary approaches that include behavioral experiments in the field and in the laboratory, neuroanatomy, in vivo electrophysiology, calcium imaging and modeling.
In his graduate studies, Tim used two - photon calcium imaging to study the neural circuits mediating locomotor behavior.