Results from each aliquot were plotted by the software onto a simultaneously run
calibration curve generated from known quantities of DYS14.
This allowed absolute quantitation to be achieved with an external
calibration curve generated from standards containing the same fixed concentration of propranolol - d7 and varied concentrations of propranolol.
Not exact matches
Provided we know the size and shape of the error in that original reading (Normal, log Normal etc) we can
generate a spread of random values around our actual reading reflecting what it might be, and then for each of those read off the age that implies, using a table that randomly selects from the (smeared out)
calibration curve at that value.
Lipid class concentrations were calculated by comparing sample peak areas and retention times against previously
generated calibration curves and retention times of known standards.