To provide further evidence that the negative effects of the low - casein diets were not limited to very young animals with unusually high needs for protein, Campbell found that the apparent loss of the ability to repair damaged tissue occurred when the 20 %
casein diet was fed during the promotion period, the same period during which it protected against the growth of pre-cancerous lesions.
Not exact matches
Subjects
were fed a liquid formula
diet of 40 % fat (corn oil), 45 % carbohydrate (glucose polymer), and 15 % protein (
casein hydrolysate) supplemented with 5.0 g iodized NaCl, 1.9 g K, and 2.5 g calcium carbonate per day, 1 mg of folic acid twice weekly, and 36 mg ferrous iron every other day.
Mice
were fed for ten weeks with a low - protein
diet containing either 10 percent
casein or a combination of 6 percent
casein and 4 percent gelatin.
Indeed, the researchers discovered that rats
fed 5 % of their
diet as
casein were generally free from cancerous growths, whereas the rats
fed 20 %
casein were riddled with»em.
And when another group of young rats on normal
feed were placed on the low -
casein diet, they stopped growing altogether.
[I] n 1945 Larsen and Heston found that the incidence of spontaneous pulmonary tumors
was doubled in strain A mice
fed low -
casein diets supplemented with cystine (the most limiting amino acid).
Dr. Campbell and one of his undergraduate students co-authored a paper in 1989 in which all the rats
were dosed with aflatoxin early on and
were all
fed 20 %
casein diets while aflatoxin
was still in their systems (7).
More clues for understanding the
casein - cancer research come from another Indian study — this one published in the late 1980s, and examining the effects of protein in aflatoxin - exposed monkeys instead of rats.14 As with Campbell's experiments, the monkeys
were fed diets containing either 5 percent or 20 percent
casein, but with one important difference: instead of
being slammed with an astronomically (and unrealistically) high dose of aflatoxin, the monkeys
were exposed to lower, daily doses — mimicking a real - world situation where aflatoxin
is consumed frequently in small amounts from contaminated foods.
After 6 months «the heart tissue and liver tissue glutathione content
were enhanced significantly above the corresponding values of the
casein diet -
fed... mice».