Sentences with phrase «cell adhesion molecules e-selectin»

Pilot studies using a variety of strategies suggested that this goal could be best achieved by stimulating primary organ cell cultures with TNF - α (10 ng / ml) and targeting cells expressing the endothelial cell adhesion molecules E-selectin and VCAM - 1 through a FACS - driven strategy.

Established cell lines exhibited several inherent endothelial properties, including the expression of constitutive and inducible levels of endothelial cell adhesion molecules E-selectin, intercellular adhesion molecule - 1, and vascular cell adhesion molecule - 1, internalization of acetylated low - density lipoprotein, and formation of loop structures on Matrigel surfaces.

The constitutive and inducible expression of the endothelial cell adhesion molecules E-selectin, ICAM - 1, and VCAM - 1 was assessed by immunohistochemistry (Figs. 4, A — C) ⇓.

Not exact matches

The constitutive expression of these endothelial cell adhesion molecules was very similar for most of the lines derived from different organs, i.e., very low expression levels of E-selectin and VCAM - 1 and more pronounced expression of ICAM - 1.

When primary cultures from these mice are stimulated with proinflammatory cytokines, the endothelial cell fraction responds by up - regulating the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1.

The selection strategy targeted cell populations expressing the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1, and proved successful in generating microvascular endothelial cell lines from a number of different organs.

To determine inducible endothelial cell adhesion molecule expression (E-selectin, VCAM - 1, and ICAM - 1), some of the chambers were given medium containing 10 ng / ml TNF - α for 4 — 6 h (these time points were selected to correlate with the known kinetics of endothelial cell adhesion molecule expression; Refs.

We therefore conducted a cross-sectional analysis to investigate the relations between magnesium intake and plasma concentrations of inflammatory and endothelial biomarkers, including CRP, IL - 6, soluble TNF - α receptor 2 (sTNF - R2), E-selectin, soluble intercellular adhesion molecule 1 (sICAM - 1), and soluble vascular cell adhesion molecule 1 (sVCAM - 1) in apparently healthy women.

In a cell - culture study, polyunsaturated fats did not alter TNF - α — mediated up - regulation of the adhesion molecules ICAM - 1, VCAM - 1, and E-selectin (34).