Sentences with phrase «cell and tissue cultures»

We exploit chick and mouse embryo animal models, combined with live imaging, cell and tissue cultures and molecular approaches.

protected animals»); studies on in vitro systems (whole perfused organs, tissue slices, cell and tissue cultures, and subcellular fractions); and human studies (including estimations of occupational and environmental exposure, postmarketing surveillance, epidemiology, and the ethical and strictly controlled use of human volunteers).

When they applied a heparanase blocker to similar lesions produced in cell and tissue culture, they healed quickly and completely.

Wanting to pursue more experimental research, he headed to the Massachusetts Institute of Technology for a postdoc to join a lab of engineers who focused on cell and tissue culture experiments.

The staff in the internationally recognised division are supported by excellent facilities in cell and tissue culture, molecular biology, novel biomaterials development and testing, physical, biological and mechanical characterisation and state - of - the - art imaging facilities, including spectroscopy, multi-photon microscopy and endoscopy.

Not exact matches

In September 2015, the two men moved to the Bay Area and started culturing cow muscle and connective tissue cells.

Animal cells are cultured from stem cells and incubated in a «bioreactor» into tissue that can be «harvested» and formed into familiar foods like meatballs, patties, and fish sticks.

The team took small pieces of ear tissue from XXY and XYY mice, cultured them, and collected connective tissue cells known as fibroblasts.

This allowed them to track changes in calcium ion concentrations in the cells around wounds in living tissue (as opposed to the cell cultures used in many previous wound response studies) and to do so with an unprecedented, millisecond precision.

Scientists are forced either to experiment on whole animals, which is expensive, raises ethical issues and may not predict effects in humans, or to perform tests on microscopic human cells found in tissue cultures, which have been altered to live forever and bear little relation to actual living, breathing people.

In the journal Biomaterials, the team reports culturing cells to make extracellular matrix (ECM) of two types and five different alignments with the strength found in natural tissue and without using any artificial chemicals that could make it incompatible to implant.

Despite being trapped and baked within a polymer, cell staining shows that the cultured cells remained alive, says Fernandez, an obvious necessity if they are to be used for artificial tissues.

The researchers — James Robl, a developmental biologist and his colleagues at the University of Massachusetts at Amherst, and Steve Stice at Advanced Cell Technology in Worcester, Massachusetts — inserted a marker gene fused with a gene for resistance to the chemical neomycin into a culture of connective tissue cells called fibroblasts.

Some of the leading non-animal tests are conducted on cell cultures, human and animal corneas from eye banks, corneal tissue cultures, and frozen corneas supplied by hospitals.

Dr. Zubair already grows such cells in his Mayo Clinic laboratory using a large tissue culture and several incubators — but only at a snail's pace.

As it can take weeks to grow human cells into intact differentiated and functional tissues within Organ Chips, such as those that mimic the lung and intestine, and researchers seek to understand how drugs, toxins or other perturbations alter tissue structure and function, the team at the Wyss Institute for Biologically Inspired Engineering led by Donald Ingber has been searching for ways to non-invasively monitor the health and maturity of cells cultured within these microfluidic devices over extended times.

In rats and tissue cultures of human nerve cells, these «beta sheet breakers» not only prevent amyloid plaques from forming, but also dissolve existing plaques.

The enzyme, called tankyrase, may prove useful for extending the lives of cultured cells grown to repair burned skin and other damaged tissue.

To test their hypothesis, the researchers harvested dermal papillae from seven human donors and cloned the cells in tissue culture; no additional growth factors were added to the cultures.

In the study, the researchers show that after two to four weeks of 3 - D culture, the resulting muscle cells form muscle fibers that contract and react to external stimuli such as electrical pulses and biochemical signals mimicking neuronal inputs just like native muscle tissue.

«However, once the dermal papilla cells are put into conventional, two - dimensional tissue culture, they revert to basic skin cells and lose their ability to produce hair follicles.

Besides displaying more natural signaling patterns, 3D cultures allow researchers to mix cell types in ways that mimic their interactions in living tissue, providing more accurate models of normal physiology and pathogenesis.

In this study, Nicchitta and his colleagues treated tissue culture cells with a stress - inducing agent called thapsigargin.

The researchers isolated the milk ducts and purified the breast - tissue cells to create a cell culture, which was then tested for different gene regulation profiles.

Part of the trouble is the ingredients: Subtle variations in tissue - culture chemicals and Matrigel, or in different stem cell lines and how they are grown first in 2 - D culture, can have a big impact on how the organoids turn out, Novitch says.

These claims were based on subjective observations of ovarian tissue and on the behavior of extremely rare ovarian cells following extensive growth in tissue culture, a procedure that is capable of «reprogramming» cells.

Together with team members Dr. Rouzanna Istvánffy and Dr. Baiba Vilne, Oostendorp used mixed cultures of tissue and stem cells to investigate how the two cell types interact.

The substance may be used for biomedical applications, ranging from cell culture and drug delivery to repairing and replacing tissue, organs and cartilage.

The researchers report they were able to transform about one in 5,000 cells — enough to get several iPS cells from a single culture dish — and then coax them to become nerve cells or heart tissue on the benchtop.

They confirmed low levels of miR - 184 expression in human glioma tissue samples and cultured cell lines as well as an increase in the expression of SND1 compared to normal brain tissue.

Their stem cells, which can mature into any type of tissue, were isolated and cultured in a dish around dots of gel - like growth medium.

We have been using cell culture models and zebrafish as an assay to determine the effects of BMP on signaling, but we also want to be able to understand what the effect of the mutation is in the tissue and organ systems.

A major challenge in creating any type of organoid is determining the unique mixture of nutrients, growth factors, and tissue culture techniques that will transform patient tumor cells into miniature tumor organoids in a petri dish.

That's an advantage for drug tests over flat - dish cell cultures currently used because it more accurately represents living tissue and more of the test cells can be kept alive by vessel - supplied nutrients.

«Our group pioneered the development of cell culture technology for harvesting large numbers of stem cells from human bone marrow and human umbilical cord blood,» Dr. Yeh said, noting that stem cells from these two sources are abundant and can be guided into different types of cells using tissue engineering.

Grima also observed this same clumping of Huntingtin protein with RanGAP1 and nuclear pore proteins to the wrong place in the cell in brain tissue and cultured brain cells derived from deceased patients with Huntington's disease.

They describe this advance in a study published in Plant Cell, Tissue and Organ Culture.

Using tissue culture models of cartilage and the meniscus, Levenston's team stresses the cells and tissues and studies what happens to them structurally and biochemically — for example, whether different genes are expressed, whether metabolism changes, or whether different types of cartilage cells respond differently to the same stress.

All human cell lines growing in tissue culture dishes, and about 95 % of cells in a human being, split evenly, resulting in daughter cells of equal size.

Researchers used cultured stem cells derived from their feline patients in the hope of reducing inflammation and promoting tissue regeneration.

In this tissue system, we can culture patient - derived megakaryocytes — the bone marrow cells that make platelets — and also endothelial cells, which are found in bone marrow and promote platelet production, to design patient - specific drug administration regimes.»

Another, previously unknown gene — when the investigators worked out its protein's structure and studied it in tissue culture — proved to do just the opposite: The protein helped the schizont leave the infected blood cell.

Considerable previous research in cell cultures has demonstrated that low doses of ionizing radiation results in «bystander» effects, in which nearby, unexposed tissues suffer cell death, mutations, and tumor - inducing growth (ScienceNOW, 7 September 2005).

Understanding and reproducing key features of bone marrow formation — and hence, the creation of blood cells and platelets in tissue culture for storage and later use — could help in treatment of a variety of medical problems.

At the time, his varied interests — in the use of skin cell culture to treat burns, in human tissue cultures, and in biopharmaceutical production — led him to do his final year, 6 - month project on culture in a bioreactor.

To overcome these limitations, we used two - color flow cytometry to identify and select microvascular endothelial cells from primary cultures obtained from different organs of mice whose tissues harbor a temperature - sensitive SV40 large T antigen (H - 2Kb - tsA58 mice; ImmortoMice).

One of the major impediments to obtaining a large number of endothelial cells from different tissues has been the inability to purify and propagate these cells in culture.

Zheng explained that in the past, measuring NMD efficiency was a cumbersome process and could be performed only on cell cultures, not directly on tissues.

Specifically, cell cultures from bone tissue were incubated in 10 % DMEM containing 10 ng / ml TNF - α and 10 μg / ml fluorescent probe of acetylated LDL, DiI - Ac - LDL (Biochemical Technologies, Cambridge, MA), for 4 h. Cells were harvested in the manner described above with the exception that during this labeling period, the rat anti-E-selectin mAb (10E9.6) conjugated to FITC replaced PE - conjugated 10E9.6.

Those doses were chosen in analogy with the prion field, in which it is very common to observe that 1 % and 0.1 % tissue homogenate containing prion protein aggregates is able to induce pathological changes both in cell culture experiments and in in vivo animal models.