Sentences with phrase «cell assays constructed»

Live cell assays constructed with genetically encoded, fluorescent biosensors can provide significant advantages over endpoint assays measured in cell lysates because functional information about the timing and location of cellular responses can be monitored in cells that are relevant to disease.

Not exact matches

The restoration of the ETS structure and function was confirmed by in - gel activity assay of complex I, which demonstrated an increase in activity to 30 — 40 % of that in WT cells in cybrids transfected by constructs containing all three modifications; it was also supported by the «marked» increase in levels of COX2 and ND6, both of which are mitochondrially - encoded ETS subunit component proteins.

The cells were cotransfected with the reporter gene constructs and related expression plasmids as indicated by using SuperFect (Qiagen) followed by transcriptional activity assay using Dual - Luciferase Assay System (Promega) according to the manufacturer's instrucassay using Dual - Luciferase Assay System (Promega) according to the manufacturer's instrucAssay System (Promega) according to the manufacturer's instruction.

These assays also recognize conserved elements within the bioengineered immuno - therapeutic constructs of CAR - T and TCRs, making them robust candidates for dual use purposes: (1) identifying and monitoring immuno - therapeutic constructs in the peripheral blood and (2) identifying and monitoring clonotypes associated with malignant B - and T - cell populations.

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